Utjecaj glukozinolata sinigrina na regulatore lipidnog metabolizma u jetri miša soja Swiss

master's thesis

• Title (english): Impact of singrin glucosinolate on the regulators of lipid metabolism in the liver of Swiss mice
• Author: Marija Matacun
• Mentor: Domagoj Đikić (mentor)
• Committee member: Domagoj Đikić (predsjednik povjerenstva)
• Committee member: Mirela Sertić Perić (član povjerenstva)
• Committee member: Draginja Mrvoš-Sermek (član povjerenstva)
• Committee member: Tajana Begović (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2023-09-20, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Glukozinolati prisutni u porodici Brassicaceae uzimaju veliku pozornost istraživačima zbog
pozitivnih svojstva koja služe prevenciji i izlječenju bolesti uzrokovanih današnjim načinom
prehrane i stilom života. Cilj je ovoga rada ispitati promjenu antioksidacijskog sastava i
transkripcijskih faktora uključenih u mehanizam kontrole metabolizma lipida u sisavaca pod
utjecajem komercijalnog sinigrina. Istraživanje je provedeno na 6 muških i 6 ženskih jedinki
miševa soja Swiss albino kojima je svakodnevno davano po 0,3 mL odnosno 10 mg sinigrina
Sigma Aldrich po gramu miša u razdoblju od 28 dana. Kontrolna je skupina sadržavala jednak
broj miševa koji su dobivali 0,3 mL fiziološke otopine. Dan nakon zadnje primjene priređeni
su homogenati tkiva jetre kojima su izmjerene antioksidacijske razine (glutation,
malondialdehid, superoksid dismutaza, katalaza) i transkripcijski faktori (PPARα, PPARγ,
PGC‒1α i ACOX) zaduženi za metabolizam lipida. Rezultati prikazuju kako se u jetri muških
jedinki događaju promjene povezane s antioksidacijskim odgovorom i u pogledu metabolizma
lipida statistički značajnim (ANOVA, p< 0,05) povećanjem malondialdehida i katalaze te
statistički značajnim smanjenjem PPARγ, PGC‒1α i ACOX1. U ženkama nigdje ne dolazi do
statističke značajnosti osim u smanjenim razinama malondialdehida. Točni razlozi odstupanja
nisu poznati, ali sigurno je kako glukozinolati odnosno njihovi produkti imaju utjecaj na
antioksidacijske i metaboličke procese u živim bićima.

Abstract (english)

Glucosinolate that are present in the Brassicaceae family are receiving a lot of attention from
researchers because of their positive properties that prevent, and cure diseases caused by today's
diet and lifestyle. The aim of this work is to examine the change in the antioxidant composition
and transcription factors involved in the control mechanism of lipid metabolism in mammals
under the influence of commercial sinigrin. The research was conducted on 6 male and 6 female
mice of the Swiss albino strain, which were given 0.3 mL or 10 mg of Sinigrin Sigma Aldrich
per gram of mouse daily for a period of 28 days. The control group contained the same number
of mice that received 0.3 mL of saline. One day after the last application, homogenates of liver
tissue were prepared and antioxidant levels (glutathione, malondialdehyde, superoxide
dismutase, catalase) and transcription factors (PPARα, PPARγ, PGC‒1α and ACOX)
responsible for lipid metabolism were measured. The results show that changes related to the
antioxidant response and lipid metabolism occur in the liver of male individuals with a
statistically significant (ANOVA, p < 0.05) increase in malondialdehyde and catalase and a
statistically significant decrease in PPARγ, PGC‒1α and ACOX1. In females, statistical
significance does not occur anywhere except in the reduced levels of malondialdehyde. The
exact reasons for the deviation are not known, but it is certain that glucosinolates or their
products have an impact on antioxidant and metabolic processes in living beings.

• Keywords: alil-izotiocijanat; antioksidans; transkripcijski faktor; glutation; lipidna peroksidacija; superoksid dismutaza
• Keywords (english): allyl-isothiocyanate; antioxidants; transcription factors; glutathione; lipid peroxidation; superoxide dismutase
• Language: croatian
• URN:NBN: urn:nbn:hr:217:628460
• Project: Number: IP-2020-02-7585; Title: Indirektni utjecaj globalnog zagrijavanja na fiziološke parametre sisavaca prehranom biljkama uzgojenim pri visokoj temperaturi; Title: Indirect Effect of Global Warming on Mammals Physiological Parameters via High Temperature-Stressed Plant Diet; Acronym: TEMPHYS; Leader: Ivana Šola; Jurisdiction: Croatia; Funder: HRZZ; Funding stream: IP
• Study programme: Title: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-09-20
• Created on: 2023-10-25 10:01:04