Investigating chromosome segregation errors in a set of human cancer cell lines by lattice light-sheet microscopy

master's thesis

• Title (croatian): Istraživanje pogrešaka segregacije kromosoma u skupu ljudskih staničnih linija raka pomoću mikroskopa s rešetkastom svjetlosnom plohom
• Author: Mario Đura
• Mentor: Iva Marija Tolić (mentor)
• Committee member: Iva Marija Tolić (predsjednik povjerenstva)
• Committee member: Maja Matulić (član povjerenstva)
• Committee member: Petra Peharec Štefanić (član povjerenstva)
• Committee member: Nada Oršolić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2023-10-19, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Chromosomal instability is a hallmark of cancer cells that furthers tumour progression by the
mis-segregation of chromosomes. Even though certain aspects of it have long been described,
novel phenomena are being established as major predictors of mitotic errors in cancers with
chromosomal instability. In this thesis, chromosomal instability was measured on 10 various
cancer cell lines through the occurrence of metaphase unaligned chromosomes, lagging
chromosomes, chromosome bridges, multipolar mitoses, micronuclei, transient multipolar
transition and other phenomena. Additionally, the co-occurrences of these phenomena were
investigated to gain insight into their correlations. The lattice light-sheet microscope allowed for
long-term live imaging of these cell lines in a substantial number of observed mitoses, enabling
the creation of a database of mitotic errors and phenomena for all cell lines in a timely manner.

Abstract (croatian)

Kromosomska nestabilnost je značajka stanica raka koja potiče progresiju tumora pomoću
nepravilne segregacije kromosoma. Iako su njeni pojedini aspekti već davno opisani, novi
fenomeni se ukazuju kao snažni čimbenici za predviđanje pogrešaka u mitozi stanica raka koje
posjeduju kromosomsku nestabilnost. U ovome radu, kromosomska nestabilnost je izmjerena u
10 raznovrsnih staničnih linija raka kroz pojavnost metafaznih neporavnatih kromosoma,
zaostajućih kromosoma, kromosomskih mostova, multipolarnih mitoza, mikronukleusa,
tranzijentne multipolarne tranzicije i ostale fenomene. Dodatno, istovremene pojavnosti ovih
fenomena su istražene kako bi se dobio uvid u njihove korelacije. Mikroskop s rešetkastom
svjetlosnom plohom je omogućio dugotrajno uživo snimanje ovih staničnih linija u značajnom
broju promatranih mitoza, što je dozvolilo relativno brzo stvaranje baze podataka mitotskih
pogrešaka i fenomena za sve stanične linije.

• Keywords: chromosomal instability; lagging chromosomes; unaligned chromosomes; micronucleus; multipolar mitosis; transient multipolar transition
• Keywords (croatian): kromosomska nestabilnost; zaostajući kromosomi; neporavnati kromosomi; mikronukleus; multipolarna mitoza
• Language: english
• URN:NBN: urn:nbn:hr:217:107191
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-10-19
• Created on: 2023-10-25 10:25:42