Detekcija gena za DNA polimerazu I bakterije Treponema pallidum lančanom reakcijom polimeraze u stvarnom vremenu

master's thesis

• Title (english): Detection of Treponema pallidum DNA polymerase I gene by real-time polymerase chain reaction
• Author: Marija Jurković
• Mentor: Ana Planinić (mentor)
• Mentor: Marin Ježić (komentor)
• Committee member: Marin Ježić (predsjednik povjerenstva)
• Committee member: Željka Vidaković-Cifrek (član povjerenstva)
• Committee member: Maja Matulić (član povjerenstva)
• Committee member: Tomislav Ivanković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2023-11-07, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Treponema pallidum je gram-negativna bakterija spiralnog izgleda koja uzrokuje sustavnu
bolest sifilis. Stopa sifilisa u svijetu je u porastu prvenstveno među muškarcima koji imaju
spolne odnose s drugim muškarcima (MSM). Sifilis povećava opasnost od prijenosa HIV-a od
dva do pet puta, a istovremena infekcija s oba uzročnika je česta. U Hrvatskoj je u 2020. g.
zabilježen porast učestalosti sifilisa od 91,4% među MSM populacijom. Cilj ovog diplomskog
rada bio je ispitati prisutnost gena za DNA polimerazu I (polA) bakterije T. pallidum u
različitim uzorcima ispitanika s različitim stadijima sifilisa te ispitati pogodnost korištenja
uzorka sline za molekularnu dijagnostiku T. pallidum. Istraživanje je provedeno na 48 uzoraka
ispitanika populacije MSM od kojih je 28 imalo i HIV-infekciju. U 9/48 (18,8%) uzoraka
detektiran je gen za DNA polimerazu I T.pallidum Najveći broj pozitivnih uzoraka su bili
brisevi ždrijela (6/26, 23,1%) dok su ostala 3/15 (20%) pozitivna uzorka predstavljali uzorci
sline. U 4/28 ispitanika s HIV-infekcijom detektirana je DNA T.pallidum. U ovom istraživanju
se po prvi puta u Hrvatskoj koristila metoda molekularne detekcije koja se pokazala
učinkovitom u dokazivanju prisutnosti uzročnika sifilisa.

Abstract (english)

Treponema pallidum is a gram-negative spiral bacterium that causes systemic disease syphilis.
The prevalence of syphilis worldwide is increasing primarily among men who have sex with
other men (MSM). Syphilis increases the risk of HIV transmission from two to five times,
while a coinfection with both of them at the same time is quite common. In 2020. a 91.4%
increase in the frequency of syphilis was recorded in MSM population in Croatia. The object
of this study was to examine the presence of the DNA polymerase I (polA) T. pallidum in
different samples with different stages of syphilis and examine the suitability of saliva for
molecular diagnosis of T. pallidum. The research was conducted on 48 samples of respondent
MSM population, 28 of whom had a HIV infection. Treponema pallidum DNA polymerase I
gene were detected in 9/48 (18,8%) samples. The largest number of positive samples were
throat swabs (6/26, 23,1%), while the others 3/15 (20%) samples were samples of saliva.
Treponema pallidum DNA was detected in 4/28 samples with HIV infection. In this research,
conducted for the first time in Croatia, molecular detection method was used, which proved to
be effective in proving the presence of the causative agent of syphilis.

• Keywords: sifilis; Treponema pallidum; lančana reakcija polimerazom
• Keywords (english): syphilis; Treponema pallidum; polymerase chain reaction
• Language: croatian
• URN:NBN: urn:nbn:hr:217:441461
• Study programme: Title: Experimental Biology-University graduate programme; Study programme type: university; Study level: graduate; Academic / professional title: sveučilišni/a magistar/magistra eksperimentalne biologije (sveučilišni/a magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-11-07
• Created on: 2023-11-22 13:43:20