Izolati vodenih plijesni (Oomycota) s kutikule slatkovodnih deseteronožnih rakova u Hrvatskoj

master's thesis

• Title (english): Oomycete (Oomycota) isolates from the cuticle of crayfish species in Croatia
• Author: Luka Petrokov
• Mentor: Ana Bielen (mentor)
• Mentor: Sandra Hudina (komentor)
• Committee member: Sandra Hudina (predsjednik povjerenstva)
• Committee member: Ivana Maguire (član povjerenstva)
• Committee member: Marin Ježić (član povjerenstva)
• Committee member: Jasna Lajtner (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2023-10-26, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Istraživanja rasprostranjenosti i utjecaja vodenih plijesni na slatkovodne deseteronožne rakove većinom su fokusirana na patogena Aphanomyces astaci, dok su u Hrvatskoj i ostalim dijelovima Europe nedovoljno istražene druge vrste vodenih plijesni koje mogu nastanjivati kutikulu rakova, iako neke od njih također mogu imati negativan utjecaj na domaćina. Cilj ovog istraživanja bio je uzgojiti i taksonomski identificirati izolate vodenih plijesni s egzoskeleta pet različitih autohtonih i invazivnih vrsta rakova u Hrvatskoj. Vodene plijesni kultivirane su iz pereiopoda i abdominalne kutikule položene na PG1 krutu hranjivu podlogu, uz precjepljivanje hifa na nove podloge do dobivanja akseničnih kultura vodenih plijesni. Identifikacija izolata do razine vrste provedena je sekvenciranjem ITS (engl. internal transcribed spacer) regije uz naknadnu filogenijsku analizu. Utvrđene su četiri vrste vodenih plijesni izolirane s kutikule dviju vrsta rakova: izolati Saprolegnia australis i Saprolegnia turfosa s kutikule vrste Pacifastacus leniusculus te izolati Aphanomyces astaci i Aphanomyces repetans s kutikule vrsta Astacus astacus i Pacifastacus leniusculus. Neke od izoliranih vodenih plijesni su saprofiti (S. turfosa, A. repetans), dok su drugi oportunisti (S. australis) ili primarni patogeni (A. astaci, uzročnik račje kuge).

Abstract (english)

Research on the distribution and effects of oomycetes on crayfish hosts is mostly focused on Aphanomyces astaci, the causative agent of the crayfish plague. In Croatia and other parts of Europe research on this topic is scarce, despite of the possibility of negative impacts on the host. The aim of this study was to cultivate and identify oomycete isolates from the exoskeleton of different native and invasive crayfish species present in Croatia. Oomycetes were cultivated from pereiopods and abdominal cuticle inoculated on PG1 solid growth media, with hyphal transfers to new media until pure cultures were obtained. Identification of the isolates to species level was performed by sequencing of the internal transcribed spacer (ITS) region followed by phylogenetic analysis. We detected four oomycete species originating from two crayfish species: Saprolegnia australis and Saprolegnia turfosa isolates originating from Pacifastacus leniusculus, Aphanomyces astaci and Aphanomyces repetans isolates originating from both Astacus astacus and Pacifastacus leniusculus. Some of the detected species are saprophytic (S. turfosa, A. repetans), while others are opportunistic (S. australis) or primary pathogens (A. astaci, the causative agent of crayfish plague).

• Keywords: Oomycota; kutikula rakova; filogenija; Saprolegniales
• Keywords (english): Oomycota; crayfish cuticle; phylogeny; Saprolegniales
• Language: croatian
• URN:NBN: urn:nbn:hr:217:747244
• Study programme: Title: Ecology and Nature Preservation-University graduate programme; Study programme type: university; Study level: graduate; Academic / professional title: sveučilišni/a magistar/magistra ekologije i zaštite prirode (sveučilišni/a magistar/magistra ekologije i zaštite prirode)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2024-10-26
• Created on: 2024-01-15 13:26:04