Terapijski učinak proantocijanidina na osteoporozu štakora prouzročenu retinoičnom kiselinom

master's thesis

• Title (english): Effect of proanthocyanidins in the treatment of osteoporosis induced by retinoic acid in rats
• Author: Edi Rođak
• Mentor: Nada Oršolić (mentor)
• Committee member: Nada Oršolić (predsjednik povjerenstva)
• Committee member: Antun Alegro (član povjerenstva)
• Committee member: Petar Kružić (član povjerenstva)
• Committee member: Duje Lisičić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2016-02-25, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Osteoporoza je bolest koštanog sustava uzrokovana poremećajem u ravnoteži između izgradnje i razgradnje koštanog tkiva u korist razgradnje. Produljenjem životnog vijeka, učestalost osteoporoze je počela rasti, što je dovelo do istraživanja patogeneze osteoporoze i razvoja lijekova ili terapeutika protiv osteoporoze. U ovom radu smo istražili učinak proantocijanidina na terapiju osteoporoze uzrokovane retinoičnom kiselinom na modelu štakora. Osteoporozu štakora smo izazvali intragastričkom primjenom 13 cis-retinoične kiseline (13cRA, 80 mg kg-1) tijekom 15 dana te potom primijenili proantocijanidin (100 mg kg-1) ili alendronat (pozitivna kontrola, 40 mg kg-1) sljedećih 15 dana. Učinkovitost proantocijanidina smo istražili praćenjem promjene mase životinja, analizom fizikalnih čimbenika bedrenih kostiju (masa, duljina i promjeri), mjerenjem mineralne gustoće metafize bedrene kosti, određivanjem udjela kalcija i fosfora u kostima te hematološkom i biokemijskom analizom. Antioksidativni i antiupalni učinak proantocijanidina smo odredili analizom relativnih masa organa, stopom lipidne peroksidacije i koncentracijama glutationa u bubregu, jetri i jajnicima. Fitoestrogeni utjecaj je procijenjen na temelju relativne mase maternice. Dobiveni rezultati pokazuju značajni antioksidativni, antiupalni, fitoestrogeni i antiosteoporotski učinak proantocijanidina na animalnom modelu osteoporoze. Izostanak negativnih posljedica proantocijanidina i njihova laka dostupnost im daje prednost u terapiji osteoporoze u odnosu na alendronat.

Abstract (english)

Osteoporosis is a disease of the skeletal system caused by disturbances in the equilibrium between formation and degradation of bone tissue in favor decomposition. With extending life expectancy, the incidence of osteoporosis began to grow, leading to increased research into pathogenesis of osteoporosis and development of drugs or therapeutics against osteoporosis. In this study, we investigated the effect of proanthocyanidins in the treatment of osteoporosis induced by retinoic acid in the rat model. We caused osteoporosis in rats by intragastric use of 13-cis retinoic acid (13cRA, 80 mg kg-1) for 15 days, and then applied proanthocyanidin (100 mg kg-1) or alendronate (positive control, 40 mg kg-1) for the next 15 days. We have explored effectiveness of proanthocyanidins by monitoring of changes in mass of animals, analysis of femur’s physical factors (weight, length and diameter), measurement of bone mineral density of femur’s metaphysis, determination of calcium and phosphorus in bone and hematological and biochemical analysis. Antioxidant and anti-inflammatory effect of proanthocyanidins was determined by analyzing the relative weight of animals, the rate of lipid peroxidation and glutathione concentrations in the kidney, liver and ovaries. Phytoestrogens impact was estimated based on the relative weight of the uterus. The results show a significant antioxidant, anti-inflammatory, phytoestrogens and antiosteoporotic effect of proanthocyanidins in animal models of osteoporosis. The absence of the negative consequences of proanthocyanidins and their easy accessibility gives them an advantage in the treatment of osteoporosis compared to alendronate.

• Keywords: proantocijanidini; osteoporoza; alendronat; retinoična kiselina
• Keywords (english): proanthocyanidins; osteoporosis; alendronate; retinoic acid
• Language: croatian
• URN:NBN: urn:nbn:hr:217:392435
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-05-12 08:17:48