Fitokemijski sastav i antioksidacijski kapacitet vodenih ekstrakata klijanaca brokule (Brassica oleracea L. convar. botrytis var. cymosa) uzgojenih pri visokoj temperaturi

master's thesis

• Title (english): Phytochemical composition and antioxidant capacity of aqueous extracts of broccoli (Brassica oleracea L. convar. botrytis var. cymosa) seedlings grown at high temperature
• Author: Eliza Fućak
• Mentor: Ivana Šola (mentor)
• Committee member: Ivana Šola (predsjednik povjerenstva)
• Committee member: Antun Alegro (član povjerenstva)
• Committee member: Ana Previšić (član povjerenstva)
• Committee member: Tomislav Ivanković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2024-02-28, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Klimatske promjene i povremeni temperaturni ekstremi utječu na razvoj biljaka za opskrbu prehrambene i ostalih industrija koje koriste biljni materijal kao sirovinu. Kako bi preživjele, biljke svoj kemijski sastav prilagođavaju okolišnim uvjetima, što može uzrokovati i promjenu u nutritivnoj vrijednosti. Svojte iz porodice kupusnjača (Brassicaceae) vrlo su zastupljene u prehrani, a sve veću popularnost stječe i tzv. “mikropovrće“ tj. klijanci zbog kratkog vremena uzgoja, manje potrebnog prostora i resursa, te visoke nutritivne vrijednosti. Brokula pripada najzastupljenijim kupusnjačama u ljudskoj prehrani, čija bi nutritivna vrijednost mogla biti ugrožena zbog porasta okolišne temperature. Stoga je cilj ovog diplomskog rada bio istražiti utjecaj simuliranih uvjeta visoke temperature okoliša na udio primarnih i specijaliziranih metabolita, te antioksidacijski kapacitet vodenih ekstrakata klijanaca brokule (Brassica oleracea L. convar. botrytis (L.) Alef. var. cymosa Duch.). Spektrofotometrijski je određen udio ukupnih fenola, flavonoida, flavonola, hidroksicimetnih kiselina, antocijana, glukozinolata, proteina i topivih šećera. Metodom tekućinske kromatografije visoke moći razlučivanja razdvojeni su, identificirani i kvantificirani L-askorbinska, sinapinska i ferulična kiselina, te flavonoidi kempferol i kvercetin. Visoka temperatura uzrokovala je povećanje udjela ukupnih fenola, flavonola i šećera, kao i koncentracije ferulične i sinapinske kiseline, kvercetina i kempferola. S druge strane, udio ukupnih flavonoida i L-askorbinske kiseline je smanjen. Metodom ABTS zabilježeno je povećanje antioksidacijskog potencijala, a metodom DPPH smanjenje.

Abstract (english)

Global warming has become one of the main challenges of modern civilizations. Climate changes and temperature extremes, affect plant production for food and other industries that use them. Plants can adapt to environment by changing phytochemical composition, which could have a significant impact on plant nutritional value. Species from Brassicaceae family are intesely represented in diet and there is also an increase in usage of „microgreens“ because of shorter growth period, resource usage and good nutritional value. As one of main Brassicaceae species present in the human diet, broccoli production could become endagered because of higher temperature environment condition, so the aim of this work was to investigate metabolic response and the impact of high temperature conditions on primary and secondary metabolites, and antioxidant capacity water extract of broccoli seedlings (Brassica oleracea L. convar. botrytis (L.) Alef. var. cymosa Duch.). The content of total phenols, flavonoids, hydroxycinnamic acids, flavonols, soluble sugars and proteins was determined spectrophotometrically. Using a high-performance-liquid-chromatography method, ferulic, sinapic and L-ascorbic acid, kaempferol and quercetin, were separated, identified and quantified. The antioxidant capacity was determined by methods ABTS, DPPH and FRAP. Higher temperature increased ferulic and sinapic acid, quercetin and kaempferol concentration, total phenols, flavonols and sugars. ABTS method showed and increase in antioxidant capacity, whereas the DPPH showed a decrease. The research also showed a decrease in flavonoids and L-ascorbic acid. The ABTS method showed an increase in the antioxidant potential, while DPPH showed a decrease.

• Keywords: fenolni spojevi; glukozinolati; HPLC; klimatske promjene; kupusnjače
• Keywords (english): Brassicaceae; climate change; glucosinolates; HPLC; phenols; spectrophotometry
• Language: croatian
• URN:NBN: urn:nbn:hr:217:009992
• Project: Number: IP-2020-02-7585; Title: Indirektni utjecaj globalnog zagrijavanja na fiziološke parametre sisavaca prehranom biljkama uzgojenim pri visokoj temperaturi; Title: Indirect Effect of Global Warming on Mammals Physiological Parameters via High Temperature-Stressed Plant Diet; Acronym: TEMPHYS; Leader: Ivana Šola; Jurisdiction: Croatia; Funder: HRZZ; Funding stream: IP
• Study programme: Title: Molecular Biology-University graduate programme; Study programme type: university; Study level: graduate; Academic / professional title: sveučilišni/a magistar/magistra molekularne biologije (sveučilišni/a magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2026-02-28
• Created on: 2024-03-08 13:23:42