Oslobađanje biološki aktivnih sastavnica iz oralnih dozirnih oblika biljnih pripravaka za liječenje upalnih bolesti crijeva u biorelevantnim medijima

doctoral thesis

• Title (english): Biorelevant dissolution of biologically active components from herbal oral dosage forms used in the treatment of inflammatory bowel disease
• Author: Snježana Zubčić
• Mentor: Daniela Amidžić Klarić (mentor)
• Committee member: Nives Galić (predsjednik povjerenstva)
• Committee member: Sanda Rončević (član povjerenstva)
• Committee member: Tomislav Bolanča (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Chemistry); Zagreb
• Defense date and country: 2024-02-29, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry
• Universal decimal classification (UDC): 54 - Chemistry. Crystallography. Mineralogy

Abstract

Kurkuminoidi, bosvelične kiseline, andrografolidi i piperin najzastupljenije su biološki aktivne sastavnice u biljnim pripravcima za liječenje upalnih bolesti crijeva. Budući da je danas primjena dodataka prehrani široko rasprostranjena i u stalnom porastu, cilj ovog rada bio je istražiti utjecaj biorelevantnih medija na in vitro profil oslobađanja biološki aktivnih sastavnica iz biljnih preparata formuliranih u čvrste oralne oblike. Osim toga, s regulatornog gledišta, cilj je bio provesti kontrolu kvalitete dozirnih oblika i teških metala u tržišno dostupnim proizvodima. Kontrola kvalitete dozirnih oblika provedena je na temelju testiranja za čvrste oblike (tablete i kapsule), a analiza metala primjenom atomske apsorpcijske spektrometrije nakon spaljivanja mikrovalnom digestijom. Živa je određena u analizatoru žive, bez prethodne obrade uzorka. Analiza biološki aktivnih sastavnica provedena je primjenom tekućinske kromatografije visoke djelotvornosti nakon oslobađanja u ispitivanim biorelevantnim medijima koji simuliraju stanje sitosti i natašte. Na temelju dobivenih rezultata svi ispitivani uzorci zadovoljili su propisane kriterije i sadržavali količinu metala ispod vrijednosti dopuštene dnevne izloženosti. Rezultati analize biološki aktivnih sastavnica odabranih biljnih vrsta pokazali su da profil in vitro oslobađanja biološki aktivne sastavnice iz oralnih biljnih pripravaka ovisi o sastavu biorelevantnog medija.

Abstract (english)

Curcuminoids, boswellic acids, andrographolides and piperine are the most abundant biologically active substances in herbal dietary supplements for the treatment of inflammatory bowel diseases. Since the use of dietary supplements is widespread and constantly increasing, this work aimed to investigate the influence of biorelevant media on the in vitro release profile of biologically active substances from oral herbal preparations. In addition, from a regulatory point of view, the goal was to analyze the quality control of solid dosage forms and heavy metals in commercially available products. The quality control of the dosage forms was carried out on the quality control tests for solid forms (tablets and capsules), and the metals were measured using atomic absorption spectrometry after incineration by microwave digestion. Mercury was determined using a mercury analyzer without pretreatment of the sample. The biologically active components were analyzed using high-performance liquid chromatography after release in tested biorelevant media that simulated feeding and fasting states. Based on the obtained results, all tested samples met the prescribed criteria and contained the amount of metal below the permissible daily exposure value. The analysis of biologically active components of selected plant species showed that the in vitro release profile of biologically active substances from oral herbal preparations depends on the composition of the biorelevant.

• Keywords: biorelevantni medij; metali; dodatci prehrani; kurkuminoidi; andrografolidi; bosvelične kiseline
• Keywords (english): biorelevant medium; metals; dietary supplements; curcuminoids; andrographolides; boswellic acids
• Language: croatian
• URN:NBN: urn:nbn:hr:217:523984
• Promotion: 2024
• Study programme: Title: Doctoral study; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica u području prirodnih znanosti (doktor/doktorica u području prirodnih znanosti)
• Type of resource: Text
• Extent: XXXIV, 90 str. ; 30 cm
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-03-22 12:47:23