MEĐUODNOS BDNF, IL-6 I POKAZATELJA OKSIDACIJSKOG STRESA PRI DEMIJELINIZACIJSKIM OŠTEĆENJIMA

doctoral thesis

• Title (english): CORRELATION OF BDNF, IL-6 AND OXIDATIVE STRESS MARKERS IN DEMYELINATING DAMAGE
• Author: Andrijana Bogoje Raspopović; MBZ: 47746243037
• Mentor: Domagoj Đikić (mentor)
• Mentor: Vida Demarin (komentor)
• Committee member: Nada Oršolić (predsjednik povjerenstva)
• Committee member: Dubravka Hranilović (član povjerenstva)
• Committee member: Mario Habek (član povjerenstva)
• Committee member: Dubravka Švob Štrac (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2024, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology
• Universal decimal classification (UDC): 577 - Biochemistry. Molecular biology. Biophysics

Abstract

BDNF, IL-6 i biljezi oskidacijskog stresa nedvojbeno imaju utjecaj na demijelinizacijski proces umultiploj sklerozi. Točni mehanizmi utjecaja na upalnu i neurodegenerativnu fazu nisu upotpunosti razjašnjeni. U ovo istraživanje je uključeno 60 ispitanika (19 muških i 41 ženskih)koji zadovoljavaju kriterije relapsno remitentne multiple skleroze (RRMS), i 66 zdravihkontrola (24 muških, 42 ženskih). Srednja dob oboljelih i zdravih je 43,5 godina. Iz krviispitanika određena je razina neurotrofina BDNF-a (proBDNF i mBDNF) i IL-6 ELISA testom,a biljega oksidacijskog stresa (MDA, GSH, katalaze i karboniliranih proteina)spektrofotometrijskom metodom, te ih se dodatno koreliralo s brojem demijelinizacijskihT1,T2/FLAIR i gadolinij imbibirajućih lezija. Utvrđena je i korelacija omjera navedenihfizioloških parametara sa stupnjem onesposobljenosti (EDSS), kognitivnim (MoCA test) idepresivnim deficitom (BDI-II upitnik). Dokazana je statistički značajna (P<0,001) negativnakorelacija serumskih razina biljega oksidacijskog stresa glutationa (GSH) na razvoj kognitivnihpromjena ispitanika s RRMS-om ispitanih MoCA testom. Ovo je prvo istraživanje ispitanika sRRMS-om temeljeno na istraživanju serumske razine glutationa na stupanj kongitivnogoštećenja ispitanog MoCA testom. Na razvoj kognitivnih promjena u ovom istraživanjuverificiranih MoCA testom, je statistički pozitivno značajno utjecao broj MR lezija(T1,T2,FLAIR) stupanj depresije, stupanj neurološke onesposobljenosti verificirane EDSSskalom, dob, te vrijednosti GSH. Ispitanici s višim vrijednostima MoCA testa su bili mlađi,imali manji stupanj neurološke onesposobljenosti, bili manje depresivni i imali su manjevrijednosti glutationa i manje demijelinizacijskih lezija na MR-u. Glutation bi mogao bitipotencijalni biljeg progresije kognitivnog deficita kod bolesnika s RRMS-om.

Abstract (english)

BDNF, IL-6 and oxidative stress markers have a distinct role on the process of demyelination in multiple sclerosis. Distinct mechanisms of influence on inflammatory and neurodegenerative phase are not clarified. 60 subjects meeting the criteria for RRMS (19 men and 41 women) and 66 healthy control subjects (24 men, 42 women) were included in the study. The average age of patients and healthy people is around 43,5 years. The levels of the neurotrophin BDNF (proBDNF and mBDNF) and IL-6 were determined from the blood of the subjects by an ELISA test and oxidative stress markers (MDA, GSH, catalase and carbonylated proteins) were determined from subjects by the spectrophotometric method and correlated with the number of demyelinating T2/FLAIR lesions and gadolininium enhanced lesions. The correlation of the ratio of the mentioned physiological parameters with the degree of disability (EDSS), cognitive (MoCA test) and depressive deficit (BDI-II questionnaire) was determined. A statistically significant (P<0.001) negative correlation of serum markers of oxidative stress levels of glutathione (GSH) on the development of cognitive changes in subjects with RRMS tested with the MoCA test was shown. This is the first study of subjects with RRMS that performed the mentioned research of serum glutathione levels on the degree of congitive damage tested by the MoCA test. The development of cognitive changes in this study, verified by the MoCA test, was statistically significantly influenced by the positive number of MR lesions (T1, T2, FLAIR), degree of depression, EDSS, age, and GSH values. Subjects with higher MoCA test values were younger, had a lower degree of neurological disability, were less depressed and had lower glutathione values and fewer demyelinating lesions on MRI. Glutathione could be a potential marker of cognitive deficit progression in patients with RRMS.

• Keywords: RRMS; biljezi oksidacijskog stresa; glutation (GSH); MoCA test
• Keywords (english): RRMS; oxidative stress markers; glutathione (GSH); MoCA test
• Language: croatian
• URN:NBN: urn:nbn:hr:217:996318
• Promotion: 2024
• Project: Number: 106-F15-00032; Title: Metabolizam kolesterola i oksidacijski stres; Acronym: BIOCIDI; Leader: Domagoj Đikić; Jurisdiction: Croatia; Funder: MZO
• Study programme: Title: Doctoral study; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti u području prirodnih znanosti (doktor/doktorica znanosti u području prirodnih znanosti)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-05-09 11:18:18