Utjecaj izloženosti α-cipermetrinu na markere oksidacijskog stresa u gravidnih ženki štakora

master's thesis

• Title (english): Effect of exposure to α-cypermethrin on markes of oxidative stress in pregnant female rats
• Author: Tena Cmrk
• Mentor: Suzana Žunec (mentor)
• Committee member: Iva Juranović Cindrić (predsjednik povjerenstva)
• Committee member: Mirta Rubčić (član povjerenstva)
• Committee member: Đani Škalamera (član povjerenstva)
• Committee member: Ivan Nemet (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Chemistry); Zagreb
• Defense date and country: 2024-05-29, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry

Abstract

Piretroidi su skupina insekticida koja ima široku primjenu u agronomiji, veterini i kućanstvu. Utvrđeno je kako piretroidi mogu djelovati kao hormonski otrovi, a posredni mehanizam između izloženosti hormonskim otrovima i njihovim učincima na ljudsko zdravlje može biti oksidacijski stres, karakteriziran poremećajem ravnoteže prooksidansa i antioksidansa. Najpoznatiji sintetski piretorid je α-cipermetrin koji se zbog svoje lipofilne prirode nakuplja u masnom tkivu, jetri i mozgu te može ući u posteljicu. Cilj ovog istraživanja bio je odrediti utjecaj izloženosti α-cipermetrinu na indukciju oksidacijskog stresa mjerenjem određenih markera u tkivima ženki štakora izloženih navedenom pesticidu u dozama 1, 10 i 19 mg kg−1 dan−1 za vrijeme gestacije. U krvi, mozgu i posteljicama ženki štakora određivani su koncentracija malondialdehida tekućinskom kromatografijom visoke djelotvornosti, a količina reaktivnih kisikovih spojeva i reduciranog glutationa te katalitička aktivnost enzima katalaze, superoksid dismutaze i glutation peroksidaze spektrofotometrijski. Poremećaj oksidacijsko- antioksidacijske ravnoteže uočen je u svim analiziranim tkivima i to ponajviše nakon izloženosti α-cipermetrinu u dozi od 1 mg kg-1. Indukcija oksidacijskog stresa u mozgu gravidnih štakorica ukazuje na moguća fiziološka oštećenja majke, a u posteljici da postoji mogućnost i za poremećaj funkcije endokrinog sustava.

Abstract (english)

Pyrethroids are a group of insecticides that are widely used in agronomy, veterinary medicine and household. It has been recognized that pyrethroids can act as endocrine disruptors, and the intermediate mechanism of exposure and effect on human health can be oxidative stress, characterized by a disturbance in the balance of prooxidants and antioxidants. The well known synthetic pyrethroid is α-cypermethrin, which due to its lipophilic nature accumulates in fatty tissue, liver and brain, but there is also the possibility of its passage through the placenta. The aim of this research is to determine the impact of exposure to α-cypermethrin on the induction of oxidative stress by measuring respective markers in the tissues of female rats exposed to the pesticide in doses of 1, 10 and 19 mg kg-1 day-1 during gestation. We analyzed the concentration of malondialdehyde by high-performance liquid chromatography, and the amount of reactive oxygen species, reduced glutathione, and the catalytic activity of the enzymes catalase, superoxide dismutase and glutathione peroxidase by spectrophotometry in the blood, brain and placentas of female rats. Disturbance of the oxidation-antioxidant balance was observed in all analyzed tissues, mostly after exposure to α-cypermethrin at a dose of 1 mg kg-1. The induction of oxidative stress in the brain of pregnant rats indicates possible physiological damage to the mother, and in the placenta, potential for disruption of the function of the endocrine system.

• Keywords: hormonski otrovi; oksidacijski stres; pesticidi; reproduktivni sustav; α-cipermetrin
• Keywords (english): hormonal toxins; oxidative stress; pesticides; reproductive system; α-cypermethrin
• Language: croatian
• URN:NBN: urn:nbn:hr:217:519604
• Study programme: Title: Graduate university studies in chemistry; specializations in: Graduate university studies in chemistry; Course: Graduate university studies in chemistry; Study programme type: university; Study level: graduate; Academic / professional title: sveučilišni/a magistar/magistra kemije (sveučilišni/a magistar/magistra kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2026-05-29
• Created on: 2024-06-05 09:32:48