Promjena signalnih putova uzrokovanih deficijencijom S-adenozinhomocistein-hidrolaze

doctoral thesis

• Title (english): Changes in signaling pathways caused by S-adenosylhomocystein hydrolase deficiency
• Author: Ivana Pavičić
• Mentor: Oliver Vugrek (mentor)
• Committee member: Neda Slade (predsjednik povjerenstva)
• Committee member: Maja Matulić (član povjerenstva)
• Committee member: Inga Urlić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2024-05-26, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology
• Universal decimal classification (UDC): 577 - Biochemistry. Molecular biology. Biophysics

Abstract

Cilj ovog doktorskog istraživanja bio je istražiti različito eksprimirane signalne putove u četiri stanične linije, odnosno SW480, MCF7, THLE3 i HEK293T, gdje je gen SAHH stabilno utišan korištenjem tehnika lentivirusnog utišavanja. Istraživanje je imalo za cilj identificirati zajedničke različito eksprimirane signalne putove u sve tri stanične linije, kao i odrediti specifične putove koji su pokazali najveću diferencijalnu ekspresiju u svakoj pojedinoj staničnoj liniji. Dodatno, istraživanje je imalo za cilj usporediti različito eksprimirane signalne putove u stanicama s utišanim genom SAHH s fibroblastima dobivenim od bolesnika s nedostatkom SAHH. Cilj je bio identificirati zajedničke putove između dva navedena eksperimentalna modela, pružajući tako uvid u potencijalne zajedničke mehanizme koji leže u osnovi SAHH-a deficijentnih staničnih procesa. Kroz sveobuhvatnu analizu i usporedbu profila ekspresije gena, istraživanje je imalo za cilj otkriti ključne putove povezane s utišavanjem SAHH gena u četiri stanične linije. Razjašnjavanjem ovih putova, istraživanje je imalo za cilj doprinijeti razumijevanju nedostatka SAHH te funkcionalne važnosti i potencijalne uključenosti u stanične procese.

Abstract (english)

The objective of this doctoral study was to investigate differentially expressed pathways in four cell lines, namely SW480, MCF7, THLE3 and HEK293T, where the gene SAHH was stably silenced using lentiviral silencing techniques. The study aimed to identify common differentially expressed pathways across all four cell lines, as well as to determine the specific pathways that exhibited the highest differential expression in each cell line individually. Additionally, the research sought to compare the differentially expressed signaling pathways SAHH-knock-down cells with fibroblast obtained from patients diagnosed with SAHH deficiency. The goal was to identify any shared pathways between the two experimental models, thereby providing insights into potential common mechanisms underlying SAHH - related cellular processes. Through comprehensive analysis and comparison of gene expression profiles, the study aimed to uncover key pathways associated with SAHH silencing in the four cell lines. By elucidating these pathways, the research aimed to contribute to the understanding of SAHH's functional significance and its potential involvement in cellular processes.

• Keywords: deficijencija SAHH-a; diferencijalno eksprimirani signalni putovi; fenotip tumorskih stanica
• Keywords (english): SAHH deficiency; differentially expressed signaling pathways; cancer cells phenotype
• Language: croatian
• URN:NBN: urn:nbn:hr:217:149185
• Promotion: 2024
• Project: Number: IP-2018-01-5632; Title: Molekularni aspekti patogenih procesa u AHCY deficijenciji; Acronym: MAPinAD; Leader: Oliver Vugrek; Jurisdiction: Croatia; Funder: Hrvatska zaklada za znanost; Funding stream: Research Projects
• Study programme: Title: Doctoral study; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti u području prirodnih znanosti (doktor/doktorica znanosti u području prirodnih znanosti)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-07-02 12:09:04