Eggshell waste transformation to calcium chloride anhydride as food-grade additive and eggshell membranes as enzyme immobilization carrier

Scientific paper - Original scientific paper

• Author: Ivica Strelec
• Author: Katarina Peranović
• Author: Marta Ostojčić
• Author: Krunoslav Aladić
• Author: Hrvoje Pavlović
• Author: Igor Đerđ
• Author: Dalibor Tatar
• Author: Nikola Maravić
• Author: Željko Skoko
• Author: Sandra Budžaki
• Author's institution: University of Zagreb; Faculty of Science; (Department of Physics)
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Physics

Abstract (english)

In continuation of our efforts to fully utilize eggshell waste (ESW), here we report the possibility of ESW transformation to calcium chloride (CaCl2) anhydride of food-grade additive purity and eggshell membranes (ESMs) as potential enzyme immobilization carriers. ESW chemical transformation by 5% (w/v) hydrochloric acid to CaCl2 solution and ESM completely devoid of the remnants of ESW calcium carbonate was performed in the constructed 15 L batch reactor during 4 h at room temperature, followed by separation of ESM from CaCl2 solution by filtration. ESW derived CaCl2 solution containing the excess hydrochloric acid was neutralized by adding calcium hydroxide, concentrated to approximately 1/8th of volume, and spray dried. Separated ESM was washed with water and acetone, dried, and ground to a size of less than 0.5mm. The ESW transformation process produced 102.42 ± 3.31 g of CaCl2 anhydrous and 2.48 ± 0.28 g of ESM per 100 g of ESW dry matter. ESW derived CaCl2 fulfilled all criteria for food-grade additive, while obtained ESM showed their suitability for Burkholderia cepacia lipase immobilization by adsorption.

• Keywords (english): eggshell waste utilization, hydrochloric acid treatment, calcium chloride anhydride, eggshell membranes, lipase immobilization
• Language: english
• Publication type: Scientific paper - Original scientific paper
• Publication status: Published
• Peer review: Peer review - international
• Publication version: Published version
• Journal title: Green Processing and Synthesis
• Numbering: vol. 13, no. 1, 20230254
• e-ISSN: 2191-9550
• DOI: https://doi.org/10.1515/gps-2023-0254
• URN:NBN: urn:nbn:hr:217:153198
• Publication: 2024-05-29
• Project: Number: IP-2020-02-6878; Title (croatian): Imobilizacija lipaza na funkcionalizirane nosače na bazi odabranih otpada iz poljoprivredno-prehrambene industrije; Title (english): Immobilization of Lipases on Functionalised Carriers Produced from Selected Agro-Food Industrial Waste; Acronym: ImoLipWaste; Leader: Sandra Budžaki; Jurisdiction: Croatia; Funding stream: Research Projects
• Document URL: https://www.degruyter.com/document/doi/10.1515/gps-2023-0254/html
• Type of resource: Text
• Access conditions: Open access
• Public note: © 2024 the author(s), published by De Gruyter. This work is licensed under the Creative Commons Attribution 4.0 International License. Received December 15, 2023; Accepted April 18, 2024; Published May 29, 2024.
• Created on: 2024-07-24 10:11:47