prikaz prve stranice dokumenta Shedding Light on Cardiac Excitation: In Vitro and In Silico Analysis of Native Ca2+ Channel Activation in Guinea Pig Cardiomyocytes using Organic Photovoltaic Devices
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Scientific paper - Original scientific paper
Shedding Light on Cardiac Excitation: In Vitro and In Silico Analysis of Native Ca2+ Channel Activation in Guinea Pig Cardiomyocytes using Organic Photovoltaic Devices
IEEE Transactions on Biomedical Engineering, 71 (2024), 6; 1980-1992. https://doi.org/10.1109/TBME.2024.3358240
Rienmüller, Theresa; Shrestha, Niroj; Polz, Mathias; Stoppacher, Sara; Ziesel, Daniel; Migliaccio, Ludovico; Pelzmann, Brigitte; Lang, Petra; Zorn-Pauly, Klaus; Langthaler, Sonja; Opančar, Aleksandar; Baumgartner, Christian; Üçal, Muammer; Schindl, Rainer; Đerek, Vedran; Scheruebel, Susanne More authors...

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Rienmüller, T., Shrestha, N., Polz, M., Stoppacher, S., Ziesel, D., Migliaccio, L. ... Scheruebel, S. (2024). Shedding Light on Cardiac Excitation: In Vitro and In Silico Analysis of Native Ca2+ Channel Activation in Guinea Pig Cardiomyocytes using Organic Photovoltaic Devices. IEEE Transactions on Biomedical Engineering, 71. (6), 1980-1992. doi: 10.1109/TBME.2024.3358240

Rienmüller, Theresa, et al. "Shedding Light on Cardiac Excitation: In Vitro and In Silico Analysis of Native Ca2+ Channel Activation in Guinea Pig Cardiomyocytes using Organic Photovoltaic Devices." IEEE Transactions on Biomedical Engineering, vol. 71, no. 6, 2024, pp. 1980-1992. https://doi.org/10.1109/TBME.2024.3358240

Rienmüller, Theresa, Niroj Shrestha, Mathias Polz, Sara Stoppacher, Daniel Ziesel, Ludovico Migliaccio, Brigitte Pelzmann, et al. "Shedding Light on Cardiac Excitation: In Vitro and In Silico Analysis of Native Ca2+ Channel Activation in Guinea Pig Cardiomyocytes using Organic Photovoltaic Devices." IEEE Transactions on Biomedical Engineering 71, no. 6 (2024): 1980-1992. https://doi.org/10.1109/TBME.2024.3358240

Rienmüller, T., et al. (2024) 'Shedding Light on Cardiac Excitation: In Vitro and In Silico Analysis of Native Ca2+ Channel Activation in Guinea Pig Cardiomyocytes using Organic Photovoltaic Devices', IEEE Transactions on Biomedical Engineering, 71(6), pp. 1980-1992. doi: 10.1109/TBME.2024.3358240

Rienmüller T, Shrestha N, Polz M, Stoppacher S, Ziesel D, Migliaccio L, and sur.. Shedding Light on Cardiac Excitation: In Vitro and In Silico Analysis of Native Ca2+ Channel Activation in Guinea Pig Cardiomyocytes using Organic Photovoltaic Devices. IEEE Transactions on Biomedical Engineering [Internet]. 2024 [cited 2024 October 07];71(6):1980-1992. doi: 10.1109/TBME.2024.3358240

T. Rienmüller, et al., "Shedding Light on Cardiac Excitation: In Vitro and In Silico Analysis of Native Ca2+ Channel Activation in Guinea Pig Cardiomyocytes using Organic Photovoltaic Devices", IEEE Transactions on Biomedical Engineering, vol. 71, no. 6, pp. 1980-1992, 2024. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:217:445483. [Accessed: 07 October 2024]

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Title (english)Shedding Light on Cardiac Excitation: In Vitro and In Silico Analysis of Native Ca2+ Channel Activation in Guinea Pig Cardiomyocytes using Organic Photovoltaic Devices
AuthorTheresa Rienmüller
AuthorNiroj Shrestha
AuthorMathias Polz
AuthorSara Stoppacher
AuthorDaniel Ziesel
AuthorLudovico Migliaccio
AuthorBrigitte Pelzmann
AuthorPetra Lang
AuthorKlaus Zorn-Pauly
AuthorSonja Langthaler
AuthorAleksandar Opančar
AuthorChristian Baumgartner
AuthorMuammer Üçal
AuthorRainer Schindl
AuthorVedran Đerek
AuthorSusanne Scheruebel
Author's institutionUniversity of Zagreb
Faculty of Science
(Department of Physics)
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Physics
Abstract (english)
Objective: This study aims to explore the potential of organic electrolytic photocapacitors (OEPCs), an innovative photovoltaic device, in mediating the activation of native voltage-gated Cav1.2 channels ( I Ca,L) in Guinea pig ventricular cardiomyocytes. Methods: Whole-cell patch-clamp recordings were employed to examine light-triggered OEPC mediated I Ca,L activation, integrating the channel's kinetic properties into a multicompartment cell model to take intracellular ion concentrations into account. A multidomain model was additionally incorporated to evaluate effects of OEPC-mediated stimulation. The final model combines external stimulation, multicompartmental cell simulation, and a patch-clamp amplifier equivalent circuit to assess the impact on achievable intracellular voltage changes. Results: Light pulses activated I Ca,L, with amplitudes similar to voltage-clamp activation and high sensitivity to the L-type Ca 2+ channel blocker, nifedipine. Light-triggered I Ca,L inactivation exhibited kinetic parameters comparable to voltage-induced inactivation. Conclusion: OEPC-mediated activation of I Ca,L demonstrates their potential for nongenetic optical modulation of cellular physiology potentially paving the way for the development of innovative therapies in cardiovascular health. The integrated model proves the light-mediated activation of I Ca,L and advances the understanding of the interplay between the patch-clamp amplifier and external stimulation devices. Significance: Treating cardiac conduction disorders by minimal-invasive means without genetic modifications could advance therapeutic approaches increasing patients' quality of life compared with conventional methods employing electronic devices.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleIEEE Transactions on Biomedical Engineering
Numberingvol. 71, no. 6, pp. 1980-1992
p-ISSN0018-9294
e-ISSN1558-2531
DOIhttps://doi.org/10.1109/TBME.2024.3358240
URN:NBNurn:nbn:hr:217:445483
Publication2024
ProjectNumber: UIP-2019-04-1753
Title (croatian): Mikro i nano-strukture za 3D opto-bioelektroniku
Title (english): Micro- and nano-structures for 3D opto-bioelectronics
Acronym: 3Doptobio
Leader: Vedran Đerek
Jurisdiction: Croatia
Funding stream: Installation Research Projects
Document URLhttps://ieeexplore.ieee.org/document/10473191/
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Public note© 2024 The Authors. This work is licensed under a Creative Commons Attribution 4.0 License. For more information, see https://creativecommons.org/licenses/by/4.0/. Date of Publication: 18 March 2024.
Created on2024-07-24 10:18:54