Praćenje bolesti račje kuge u populacijama invazivne vrste bodljobradog raka (Faxonius limosus) u Hrvatskoj

master's thesis

• Title (english): Monitoring of crayfish plague in populations of the invasive spiny-cheek crayfish (Faxonius limosus) in Croatia
• Author: Mia Bednjanić
• Mentor: Sandra Hudina (mentor)
• Mentor: Ana Bielen (komentor)
• Committee member: Sandra Hudina (predsjednik povjerenstva)
• Committee member: Ivana Maguire (član povjerenstva)
• Committee member: Jasna Lajtner (član povjerenstva)
• Committee member: Zoran Marčić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2024-07-12, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Bodljobradi rak (Faxonius limosus) je invazivna vrsta koja predstavlja značajnu prijetnju autohtonim populacijama rakova u Europi. Potječe iz Sjeverne Amerike te se brzo širi na nova područja gdje ulazi u kompeticiju za resurse s autohtonim vrstama te prenosi bolesti poput račje kuge. Račja kuga, uzrokovana patogenom Aphanomyces astaci, ima devastirajući učinak na autohtone vrste rakova, što naglašava potrebu za stalnim monitoringom ovog patogena. Na području istočne Hrvatske, Faxonius limosus širi svoj areal u Dunavu i Dravi te ugrožava nativne vrste rakova Astacus astacus i Pontastacus leptodactylus. Dosadašnja istraživanja pokazala su prisutnost patogena u populacijama F. limosus, ali bez uspješne genotipizacije zbog niske koncentracije DNA patogena u uzorcima. Cilj ovog istraživanja bio je utvrditi prisutnost patogena Aphanomyces astaci u recetnim uzorcima bodljobradog raka iz istočne Hrvatske. Koristeći PCR metodu patogen nije detektiran u 11 analiziranih jedinki Faxonius limosus, ali je detektiran u uzorku autohtone vrste Astacus astacus sakupljene u istočnom dijelu Republike Hrvatske. Genotipizacija patogena A. astaci dobivenog iz vrste A. astacus nije bila uspješna, vjerojatno zbog preniske koncentracije DNA patogena u uzorku. U nastavku istraživanja potrebno je koristiti osjetljivije molekularne metode za pouzdaniju detekciju patogena.

Abstract (english)

The spiny-cheek crayfish (Faxonius limosus) is an invasive species that poses a significant threat to native crayfish populations in Europe. Originating from North America, it rapidly spreads to new areas, competing with native species for resources and transmitting diseases such as crayfish plague. Crayfish plague, caused by the pathogen Aphanomyces astaci, has a devastating impact on native crayfish species, highlighting the need for constant monitoring of this pathogen. In eastern Croatia, Faxonius limosus is expanding its range in the Danube and Drava rivers, threatening the native crayfish species Astacus astacus and Pontastacus leptodactylus. Previous research has shown the presence of the pathogen in populations of F. limosus, but without successful genotyping due to the low concentration of pathogen DNA in the samples. The aim of this study was to determine the presence of the pathogen Aphanomyces astaci in recent samples of spiny-cheek crayfish from eastern Croatia. Using the PCR method, the pathogen was not detected in 11 analyzed individuals of Faxonius limosus, but it was detected in a sample of the native species Astacus astacus collected in the eastern part of the Republic of Croatia. Genotyping of the A. astaci pathogen obtained from A. astacus was unsuccessful, probably due to the low concentration of pathogen DNA in the sample. Further research needs to use more sensitive molecular methods for more reliable detection of the pathogen.

• Keywords: Aphanomyces astaci; genotipizacija; autohtone vrste; detekcija patogena
• Keywords (english): Aphanomyces astaci; genotyping; native species; pathogen detection
• Language: croatian
• URN:NBN: urn:nbn:hr:217:754514
• Study programme: Title: Ecology and Nature Preservation-University graduate programme; Study programme type: university; Study level: graduate; Academic / professional title: sveučilišni/a magistar/magistra ekologije i zaštite prirode (sveučilišni/a magistar/magistra ekologije i zaštite prirode)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-07-29 12:05:58