Deconstructing the role of myosin contractility in force fluctuations within focal adhesions

Scientific paper - Original scientific paper

• Author: Debsuvra Ghosh
• Author: Subhadip Ghosh
• Author: Abhishek Chaudhuri
• Author's institution: University of Zagreb; Faculty of Science; (Department of Physics)
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Physics

Abstract (english)

Force fluctuations exhibited in focal adhesions that connect a cell to its extracellular environment point to the complex role of the underlying machinery that controls cell migration. To elucidate the explicit role of myosin motors in the temporal traction force oscillations, we vary the contractility of these motors in a dynamical model based on the molecular clutch hypothesis. As the contractility is lowered, effected both by changing the motor velocity and the rate of attachment/detachment, we show analytically in an experimentally relevant parameter space, that the system goes from decaying oscillations to stable limit cycle oscillations through a supercritical Hopf bifurcation. As a function of the motor activity and the number of clutches, the system exhibits a rich array of dynamical states. We corroborate our analytical results with stochastic simulations of the motor-clutch system. We obtain limit cycle oscillations in the parameter regime as predicted by our model. The frequency range of oscillations in the average clutch and motor deformation compares well with experimental results.

• Keywords (english): myosin contractility; focal adhesions; force fluctuations
• Language: english
• Publication type: Scientific paper - Original scientific paper
• Publication status: Published
• Peer review: Peer review - international
• Publication version: Published version
• Journal title: Biophysical Journal
• Numbering: vol. 121, no. 9, pp. 1753-1764
• p-ISSN: 0006-3495
• e-ISSN: 1542-0086
• DOI: https://doi.org/10.1016/j.bpj.2022.03.025
• URN:NBN: urn:nbn:hr:217:227254
• Publication: 2022-03
• Project: Number: KK.01.1.1.01.0004; Title (croatian): Provedba vrhunskih istraživanja u sklopu Znanstvenog centra izvrsnosti za kvantne i kompleksne sustave te reprezentacije Liejevih algebri; Title (english): Implementation of top research within the Scientific Center of Excellence for Quantum and Complex Systems, and Representations of Lie Algebras; Acronym: QuantiXLie; Leader: Hrvoje Buljan; Jurisdiction: eu; Funding stream: European Regional Development Fund
• Document URL: https://www.sciencedirect.com/science/article/pii/S0006349522002351
• Type of resource: Text
• Access conditions: Open access
• Public note: © 2022 Biophysical Society. Submitted August 21, 2021, and accepted for publication March 21, 2022.Published 3 May 2022.
• Created on: 2024-08-21 20:15:44