Polifazna karakterizacija sojeva cijanobakterija iz bentičkih i epibiotskih staništa istočnog Jadranskog mora

master's thesis

• Title (english): Polyphasic characterization of cyanobacterial strains from benthic and epibiotic habitats from the eastern Adriatic Sea
• Author: Ela Pahor
• Mentor: Sunčica Bosak (mentor)
• Mentor: Lucija Kanjer (komentor)
• Committee member: Sunčica Bosak (predsjednik povjerenstva)
• Committee member: Petar Žutinić (član povjerenstva)
• Committee member: Sandra Hudina (član povjerenstva)
• Committee member: Marija Gligora Udovič (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2024-07-15, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Cijanobakterije su fotolitoautotrofni prokarioti koje nalazimo široko rasprostranjene diljem svijeta u različitim staništima zahvaljujući njihovoj izrazitoj sposobnosti prilagodbe. Njihova prva sistematska podjela temeljila se isključivo na morfološkim značajkama, međutim uključivanjem molekularnih metoda i filogenetskih analiza došlo je do znatnih promjena u klasifikaciji i imenovanju taksona. Najboljim se pristupom smatra polifazna karakterizacija taksona koja uključuje analiziranje većeg broja čimbenika kao što su filogenetske, morfološke, fiziološke i ekološke značajke. Cilj ovog rada bio je izolirati i polifaznim pristupom opisati bentičke taksone iz epilitskih staništa zona supralitorala i mediolitorala istočnog Jadranskog mora te epibiotske taksone cijanobakterija s oklopa glavatih želvi. Izolirane su ukupno 34 laboratorijske kulture koje su na temelju morfologije svrstane u 4 osnovna morfotipa. Na temelju morfoloških i filogenetskih analiza koje su uključivale 16S rDNA, 28 sojeva izoliranih iz bentičkih staništa su svrstani u rodove Sodalinema, Salileptolyngbya i Nunduva, te su prvi put zabilježeni za područje Jadranskog mora. Svih 6 epibiotskih sojeva izoliranih s oklopa glavatih želvi su svrstani u rod Leptothoe, koji je prethodno zabilježen u metagenomskim istraživanjima oklopa morskih kornjača, međutim u ovom radu su prvi put pripadnici izolirani u laboratorijske kulture. Za potpunu i točnu karakterizaciju sojeva, u budućim se istraživanjima preporuča uključiti dodatne genske markere u filogenetsku analizu, kako bi se riješile nedoumice u identifikaciji i klasifikaciji te pridonijelo opisivanju novih taksona izoliranih u ovome radu.

Abstract (english)

Cyanobacteria are photolithoautotrophic organisms inhabiting different ecosystems all over the world thanks to their exceptional adaptability. Classification and taxonomy of cyanobacteria were at first based solely on their morphology and have changed significantly with the introduction of molecular methods and phylogenetic analyses. Polyphasic characterization is considered the best approach because it includes the analysis of several parameters, like phylogenetic, morphological, physiological and ecological traits of cyanobacteria. The aim of this study was to isolate and describe benthic taxons of cyanobacteria from epilithic habitats of supralittoral and mediolittoral zones of the eastern Adriatic Sea and epibiotic taxons of cyanobacteria from the carapace of loggerhead sea turtles, using polyphasic approach. A total of 34 laboratory cultures were isolated and classified into 4 basic morphotypes based on their morphology. Based on morphological and phylogenetic analyses that included 16S rDNA, 28 strains isolated from benthic habitats were classified into the genera Sodalinema, Salileptolyngbya and Nunduva, and were recorded for the first time in the Adriatic Sea. All 6 epibiotic strains isolated from the carapace of loggerhead sea turtles were classified in the genus Leptothoe, previously discovered on the carapace of sea turtles using metagenomics, and in this study were isolated into laboratory cultures for the first time. For future research a complete and accurate characterization of strains, including additional genetic markers in the phylogenetic analysis, is recommended to resolve the doubts in identification and classification and attribute to the description of taxons new to science.

• Keywords: Leptothoe; Nunduva; Salileptolyngbya; Sodalinema; Caretta caretta; supralitoral
• Keywords (english): Leptothoe; Nunduva; Salileptolyngbya; Sodalinema; Caretta caretta; supralittoral
• Language: croatian
• URN:NBN: urn:nbn:hr:217:907914
• Project: Number: UIP-2017-05-5635; Title: Mikrobiom glavate želve (Caretta caretta): uvid u epizoičke i endozoičke zajednice; Title: Loggerhead sea turtle (Caretta caretta) microbiome: insight into endozoic and epizoic communities; Acronym: TurtleBIOME; Leader: Sunčica Bosak; Jurisdiction: Croatia; Funder: Hrvatska zaklada za znanost; Funding stream: Installation Research Projects
• Study programme: Title: Ecology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra ekologije i zaštite prirode (magistar/magistra ekologije i zaštite prirode)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-09-03 12:27:23