Mehanizmi popravka DNA kod arheja

undergraduate thesis

• Title (english): Mechanisms of DNA repair in Archaea
• Author: Filipa Stanojević
• Mentor: Ivana Ivančić Baće (mentor)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2024-09-06, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Oštećenja DNA uzrokovana endogenim i egzogenim čimbenicima utječu na vijabilnost organizama, a time i na opstanak vrste. Zahvaljujući mnogim mehanizmima popravka DNA oštećenja DNA se mogu efikasno popraviti. Arheje spadaju u treću domenu života, smatraju se precima eukariota i poznate su po mogućnosti života u ekstremnim uvjetima okoliša. Zbog problematičnog uzgoja u laboratoriju nije se mnogo znalo o načinima popravka DNA u njima. Razvojem sekvenciranja DNA i metagenomike istraživanja su znatno olakšana i otkrivene su brojne sličnosti s prokariotskim i eukariotskim genima. U ovome radu opisat ću tri mehanizma popravka DNA: popravak krivo sparenih baza, ekscizijski popravak nukleotida i popravak dvolančanih lomova DNA homolognom rekombinacijom. Ukazat ću na složenost puteva kojima se mogu odvijati različiti mehanizmi popravka, umreženosti proteina uključenih u popravak DNA te evolucijski i biotehnološki značaj istraživanja popravka DNA u arhejama.

Abstract (english)

DNA damage caused by endogenous and exogenous factors, if left unrepaired, can have a negative effect on organismal fitness and at last the survival of species. Multiple mechanisms of DNA repair are vital for efficient repair of damaged DNA. Archeae, which belong to the third domain od life are known for inhabiting a very broad range of extreme environments. They are believed to be eukaryotes’ ancestors. However, because of the complexity of cultivating archaeal species in the laboratory, little was known about their DNA repair mechanisms. With the rapid development of DNA sequencing and metagenomics, the research of archaea has been made significantly easier resulting in the discovery of many similarities between archaeal and prokaryotic and eukaryotic genes. With research being made easier, mysteries held by the third domain of life have started to be unraveled. Throughout this thesis, I will review three DNA repair mechanisms: mismatch repair, nucleotide excision repair, and double-strand break repair dependent on homologous recombination. I will also highlight the complexity of these DNA repair mechanisms, the cooperation between proteins involved in DNA repair, and the significance of researching DNA repair pathways in archaea.

• Keywords: arheje; DNA; MMR; homologna rekombinacija; NER
• Keywords (english): archaea; DNA; MMR; homologous recombination; NER
• Language: croatian
• URN:NBN: urn:nbn:hr:217:545220
• Study programme: Title: Molecular biology-University undergraduate programme; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) molekularne biologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-09-12 17:08:38