Procjena 3-hidroksi-2-piridinskih oksima kao mogućih protutumorskih lijekova

master's thesis

• Title (english): Assessment od 3-hydroxy-2-pyridine oximes as potential anticancer drugs
• Author: Matea Jurić
• Mentor: Maja Katalinić (mentor)
• Mentor: Domagoj Đikić (komentor)
• Committee member: Domagoj Đikić (predsjednik povjerenstva)
• Committee member: Romana Gračan (član povjerenstva)
• Committee member: Željka Vidaković-Cifrek (član povjerenstva)
• Committee member: Vesna Benković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2024-09-17, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Piridinijevi oksimi se preteņno istraņuju kao protuotrovi kod trovanja organofosfatima, no prema novim istraņivanjima utvrĎeno je da aktiviraju apoptozu u različitim vrstama stanica ńto ih čini zanimljivima i za druge aspekte istraņivanja. U ovom radu ispitan je učinak 3-hidroksi-2-piridinskih oksima prema stanicama raka dojke (MDA-MB-231 i MCF-7) i malignog glioblastoma (U251) u svrhu odreĎivanja njihovog mogućeg protutumorskog potencijala. Testirani oksimi bili su citotoksični za stanice raka dojke i, u manjoj mjeri, za stanice glioblastoma unutar razdoblja izlaganja. Stanična linija MCF-7 bila je najosjetljivija, na koju su sva tri oksima utjecala ovisno o vremenu izloņenosti. Iako je integritet membrane stanica odrņan nakon tretmana, testirani spojevi su utjecali na potencijal mitohondrijske membrane i aktivirali kaspazu 9, odnosno apoptozu posredovanu mitohondrijima. Sveukupno, ovi rezultati sugeriraju da bi istraņivani oksimi mogli posluņiti kao obećavajuća polazna točka za razvoj ciljanih terapija, posebno za rak dojke

Abstract (english)

Pyridinium oximes are mainly studied as antidotes for organophosphate poisoning, but according to new research it has been shown that they activate apoptosis in different types of cells, which makes them interesting for other aspects of research. In this thesis, the effect of 3-hydroxy-2-pyridine oximes on breast cancer cells (MDA-MB-231 and MCF-7) and malignant glioblastoma (U251) was examined in order to determine their possible anticancer potential. The tested oximes were cytotoxic to breast cancer cells and, to a lesser extent, to glioblastoma cells within the exposure period. The MCF-7 cell line was the most sensitive, being affected by all three oximes depending on the exposure time. Although cell membrane integrity was maintained after the treatment, the tested compounds affected the mitochondrial membrane potential and activated caspase 9, which is mitochondria-mediated apoptosis. Overall, these results suggest that the investigated oximes could serve as a promising starting point for the development of targeted therapies, especially for breast cancer.

• Keywords: apoptoza; citotoksičnost; rak dojke; glioblastom
• Keywords (english): apoptosis; cytotoxicity; breast cancer; glioblastoma
• Language: croatian
• URN:NBN: urn:nbn:hr:217:364641
• Study programme: Title: Experimental Biology-University graduate programme; Study programme type: university; Study level: graduate; Academic / professional title: sveučilišni/a magistar/magistra eksperimentalne biologije (sveučilišni/a magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-09-22 10:20:50