Simetrični poliini izvedeni iz bis-triarilamina kao fluorescentni senzori za DNA i RNA

master's thesis

• Title (english): Symmetrical polyynes derived from bis-triarylamine as fluorescence sensor for DNA and RNA
• Author: Nina Vukadinović
• Mentor: Dijana Pavlović Saftić (mentor)
• Committee member: Iva Juranović Cindrić (predsjednik povjerenstva)
• Committee member: Đani Škalamera (član povjerenstva)
• Committee member: Mirta Rubčić (član povjerenstva)
• Committee member: Ivan Nemet (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Chemistry); Zagreb
• Defense date and country: 2024-10-16, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry

Abstract

Fluorescentne probe za analitičke svrhe su ključne za napredak u gotovo svim područjima prirodnih znanosti, a vrlo često pronalaze primjenu u specifičnoj detekciji polinukleotida. U okviru ovog rada provedena je spektroskopska karakterizacija pet spojeva izvedenih iz bis-triarilamina primjenom UV/Vis i fluorescencijske spektroskopije. Ispitan je utjecaj supstituenata na afinitet i način vezanja na prirodnu DNA te sintetske molekule DNA i RNA. Obradom fluorimetrijskih titracija pomoću Scatchardove jednadžbe metodom nelinearne regresijske analize dobivene su konstante stabilnosti kompleksa proba s polinukleotidima. Eksperimenti temperaturnog mekšanja polinukleotida i titracije metodom cirkularnog dikroizma su pokazali visok afinitet spojeva prema polinukleotidima, bez značajne selektivnosti prema specifičnim sekvencama. Dodatak ovih spojeva dvolančanim molekulama obično je rezultirao slabijom (de)stabilizacijom heliksa, osim jednog spoja koji je blago stabilizirao određeni tip DNA. Dobiveni rezultati ukazuju na potencijal ovih spojeva kao fluorescentnih senzora u biološkim istraživanjima.

Abstract (english)

Fluorescent probes for analytical purposes are crucial for advancements in nearly all fields of natural sciences and are often used for the specific detection of polynucleotides. This study involved the spectroscopic characterization of five compounds derived from bis-triarylamines using UV/Vis and fluorescence spectroscopy. The influence of substituents on the affinity and binding mode to natural DNA and synthetic DNA and RNA molecules was investigated. Analysis of fluorimetric titrations using the Scatchard equation with nonlinear regression methods provided stability constants for the complexes with polynucleotides. Experiments on the thermal melting of polynucleotides and circular dichroism titrations demonstrated a high affinity of the compounds for polynucleotides, with no significant selectivity towards specific sequences. The addition of these compounds to double-stranded molecules generally resulted in weaker stabilization of the helix, except for one compound, which slightly stabilized a specific type of DNA. The results indicate the potential of these compounds as fluorescent sensors in biological research.

• Keywords: bis-triarilamini; cirkularni dikroizam; DNA; fluorescentne probe; UV/Vis
• Keywords (english): bis-triarylamines; circular dichroism; DNA; fluorescent probes; UV/Vis
• Language: croatian
• URN:NBN: urn:nbn:hr:217:725000
• Project: Number: IP-2022-10-9829; Title: Novi peptid-oligonukleotid-kromofor konjugati za biokemijsku dijagnostiku i bioaktivnost; Title: New peptide-oligonucleotide-chromophore conjugates for biochemical diagnostics and bioactivity; Acronym: POC-Sens-Bioact; Leader: Ivo Piantanida; Jurisdiction: Croatia; Funder: Hrvatska zaklada za znanost; Funding stream: Research Projects
• Study programme: Title: Graduate university studies in chemistry; specializations in: Graduate university studies in chemistry; Course: Graduate university studies in chemistry; Study programme type: university; Study level: graduate; Academic / professional title: sveučilišni/a magistar/magistra kemije (sveučilišni/a magistar/magistra kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-10-24 12:52:07