Antimikrobnim peptidima funkcionalizirane DNA nanopore s antibakterijskim učinkom

master's thesis

• Title (english): DNA nanopores functionalized with antimicrobial peptides exhibiting antibacterial effect
• Author: Petra Katalinić
• Mentor: Ivo Crnolatac (mentor)
• Committee member: Iva Juranović Cindrić (predsjednik povjerenstva)
• Committee member: Ines Primožič (član povjerenstva)
• Committee member: Biserka Prugovečki (član povjerenstva)
• Committee member: Adriana Kenđel (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Chemistry); Zagreb
• Defense date and country: 2024-10-09, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry

Abstract

Bakterijska rezistencija predstavlja ozbiljan problem u suvremenoj medicini. Ona omogućuje bakterijama da se razvijaju unatoč prisutnosti antibiotika, što dovodi do povećanja troškova zdravstvene zaštite te u krajnjem slučaju, smrtnosti. Zbog nedostatka lijekova, kao alternativno rješenje, istražuju se antimikrobni peptidi koji potencijalno djeluju protiv raznih mikroorganizama. Spomenuti peptidi, anoplin i indolicidin, zbog svoje amfipatske strukture, mogu destabilizirati bakterijske membrane. Da bi se povećao terapeutski učinak, istražuje se konjugacija peptida s oligonukleotidima. U ovom istraživanju fokus je stavljen na sintezu i analizu konjugata peptida i oligonukleotida pomoću klik-kemijske reakcije. Sintetizirano je 14 spojeva, uključujući konjugate peptida i oligonukleotida te komplekse različitih oligonukleoida. Spojevi su okarakterizirani UV/Vis i fluorescencijskom spektrofotometrijom te spektrometrijom cirkularnog dikroizma. Sinteza je praćena gel-elektroforezom, a završni produkti podvrgnuti su mikrobiološkom testiranju. Autentičnost produkata sinteze i završnih produkata nije potvrđena strukturnom analizom. Potrebna je analiza produkata klik sinteze i smatanja konjugata primjenom spektrometrije masa.

Abstract (english)

Bacterial resistance represents a serious problem in modern medicine. It allows bacteria to prosper, despite the presence of antibiotics, leading to increased healthcare costs and, ultimately, mortality. Due to the lack of drugs, as an alternative solution, antimicrobial peptides are being explored for their potential activity against various microorganisms. These peptides, anoplin, and indolicidin, can destabilize bacterial membranes due to their amphipathic character. To enhance therapeutic efficacy and stability, our research is focusing on conjugating peptides with oligonucleotides, which provides more structural stability while allowing precise targeting. This study focuses on the synthesis and analysis of peptide-oligonucleotide conjugates using click chemistry. 14 compounds were synthesized, including conjugates of peptides and oligonucleotides and complexes of different oligonucleotides. The compounds were characterized by UV/Vis and fluorescence spectrophotometry and circular dichroism spectrometry. The synthesis was monitored by gel electrophoresis, and the final products were subjected to microbiological testing. The authenticity of the conjugates and the final products could not be confirmed with structural analysis. Therefore, the products of the click-synthesis and the annealed conjugates should be analysed by mass spectroscopy.

• Keywords: anoplin; antimikrobni peptidi; bakterije; indolicidin; oligonukleotidi
• Keywords (english): anoplin; antimicrobial peptides; bacteria; indolicidin; oligonucleotides
• Language: croatian
• URN:NBN: urn:nbn:hr:217:023984
• Study programme: Title: Graduate university studies in chemistry; specializations in: Graduate university studies in chemistry; Course: Graduate university studies in chemistry; Study programme type: university; Study level: graduate; Academic / professional title: sveučilišni/a magistar/magistra kemije (sveučilišni/a magistar/magistra kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-11-07 15:11:55