OSTEOINDUKTIVNI UČINAK DEKSAMETAZONA NA DIFERENCIJACIJU LJUDSKIH MEZENHIMSKIH MATIČNIH STANICA U PEPTIDNOM HIDROGELU IN VITRO I IN VIVO

doctoral thesis

• Title (english): OSTEOINDUCTIVE EFFECT OF DEXAMETHASONE ON IN VITRO AND IN VIVO HUMAN MESENCHYMAL STEM CELLS DIFFERENTIATION IN PEPTIDE HYDROGEL
• Author: Lidija Požgaj
• Mentor: Inga Urlić (mentor)
• Committee member: Maja Matulić (predsjednik povjerenstva)
• Committee member: Vesna Eraković Haber (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2024-08-08, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology
• Universal decimal classification (UDC): 577 - Biochemistry. Molecular biology. Biophysics

Abstract

Koštana oboljenja postaju veliki socioekonomski problem u kontekstu starenja stanovništva. Stoga postoji sve veća potreba za novim metodama liječenja koštanih oboljenja poput regenerativne ortopedije kojoj je glavni cilj obnova oštećenog koštanog tkiva između ostalog i ugradnjom tzv. bioloških nosača. Ranija istraživanja pokazuju da deksametazon u kulturama stanica potiče diferencijaciju ljudskih mezenhimskih matičnih stanica u osteoblaste. Glavni cilj ovog doktorskog rada bio je ispitati može li se deksametazon koristiti kao biosignalna molekula, ugrađena unutar hidrogel nosača RADA 16-I te posjeduje li osteogeni učinak na diferencijaciju ljudskih mezenhimskih matičnih stanica u dinamičnom sustavu perfuzijskog bioreaktora i modelu in vivo (ektopičan rast kosti). Rezultati pokazuju da deksametazon ugrađen unutar hidrogela RADA 16-I posjeduje osteogeni učinak i da ljudske mezenhimske matične stanice diferenciraju u ostoblaste ovisno o koncentraciji deksametazona. Nosač koji kombinira hidrogel RADA 16-I, deksametazon i ljudske mezenhimske matične stanice mogao bi imati veliki potencijal za primjenu u području regenerativne ortopedije i liječenju koštanih oboljenja u budućnosti.

Abstract (english)

Bone diseases are becoming a major socioeconomic problem due to the aging of the population.
Therefore, the need for new methods in the field of regenerative orthopaedics is increasing. The main goal of regenerative orthopaedics is to restore damaged bone tissue using various principles, like biological scaffolds. Prior studies shows that dexamethasone in cell culture promotes the differentiation of human mesenchymal stem cells into osteoblasts.
The main goal of this doctoral thesis is to demonstrate that dexamethasone can be used as a bioactive molecule, embedded within the RADA 16-I hydrogel carrier, and that it possesses osteogenic potential for the differentiation of human mesenchymal stem cells, both in a dynamic perfusion bioreactor system and an in vivo model for ectopic bone growth.
The results show that dexamethasone incorporated within the RADA 16-I hydrogel possesses osteogenic potential and that human mesenchymal stem cells differentiate into osteoblasts depending on the concentration of dexamethasone. A scaffold that combines hydrogel RADA 16-I, dexamethasone and human mesenchymal stem cells could have great potential for application in the field of regenerative orthopaedics and in the treatment of bone diseases in the future.

• Keywords: kost; deksametazon; hidrogel; ljudske mezenhimske matične stanice; osteoblast; bioreaktor
• Keywords (english): bone; dexamethasone; hydrogel; human mesenhymal stem cells; osteoblasts; biorector
• Language: croatian
• URN:NBN: urn:nbn:hr:217:747640
• Promotion: 2024
• Study programme: Title: Doctoral study; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti u području prirodnih znanosti (doktor/doktorica znanosti u području prirodnih znanosti)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-12-02 10:22:05