ANALIZA PROTEINSKOG PROFILA U STANICAMA INTRAEPITELNIH LEZIJA VRATA MATERNICE

doctoral thesis

• Title (english): PROTEIN PROFILE ANALYSIS OF CELLS FROM INTRAEPITHELIAL CERVICAL LESIONS
• Author: Ena Pešut
• Mentor: Ivan Sabol (mentor)
• Committee member: Maja Matulić (predsjednik povjerenstva)
• Committee member: Rosa Karlić (član povjerenstva)
• Committee member: Rajko Fureš (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2024-10-21, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology
• Universal decimal classification (UDC): 577 - Biochemistry. Molecular biology. Biophysics

Abstract

Rak vrata maternice četvrti je po učestalosti i smrtnosti u svijetu, a prethode mu prekancerozne intraepitelne lezije povezane s infekcijom ljudskim papiloma virusom. Cilj ovog istraživanja bio je analizirati proteinski profil stanica ovisno o stupnju prekancerozne intraepitelne lezije i prisutnosti ljudskog papiloma virusa. U ovom istraživanju je na brisevima vrata maternice s prekanceroznim intraepitelnim lezijama utvrđena prisutnost ljudskog papiloma virusa lančanom reakcijom polimerazom, a proteinski profil masenom citometrijom i imunocitokemijom. Na bioptatima vrata maternice utvrđen je proteinski profil imunohistokemijom. Rezultati su pokazali da je masena citometrija prikladna za uspješnu analizu proteinskog profila na brisevima vrata maternice. Ekspresija proteina Ck18, Ck8, CD63, CD71, KLF4, LRIG1, E-kadherin, Nanog, p53, Ck17, MCM2, MMP7 i CD29 razlikovala se s obzirom na težinu stupnja prekancerozne lezije i prisutnost HPV-a.

Abstract (english)

Cervical cancer is the fourth most common cancer in the world by incidence and mortality, and is preceded by precancerous intraepithelial lesions associated with human papillomavirus (HPV) infection. The aim of this study was to analyze the protein profile of cells depending on the stage of the precancerous intraepithelial lesion and the presence of HPV. In this study, the presence of HPV was determined on cervical swabs with precancerous intraepithelial lesions by polymerase chain reaction, and the protein profile was established by mass cytometry and immunocytochemistry. The protein profile of cervical biopsies was determined by immunohistochemistry. The results showed that mass cytometry was suitable for the successful analysis of the protein profile on cervical swabs. The expression of proteins Ck18, Ck8, CD63, CD71, KLF4, LRIG1, E-cadherin, Nanog, p53, Ck17, MCM2, MMP7, and CD29 differed with regard to the severity of the precancerous lesion and the presence of HPV.

• Keywords: rak vrata maternice; HPV; CyTOF; SIL; CIN; LBC; protein
• Keywords (english): cervical cancer; HPV; CyTOF; SIL; CIN; LBC; protein
• Language: croatian
• URN:NBN: urn:nbn:hr:217:239709
• Promotion: 2024
• Project: Number: IP-2019-04-3403; Title: Multifaktorna analiza HPV inficiranih stanica u nastanku raka vrata maternice; Title: Multifactor analysis of HPV infected cells in cervical cancer development; Acronym: MACCD; Leader: Ivan Sabol; Jurisdiction: Croatia; Funder: Hrvatska zaklada za znanost; Funding stream: Research Projects
• Study programme: Title: Doctoral study; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti u području prirodnih znanosti (doktor/doktorica znanosti u području prirodnih znanosti)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-12-02 12:04:41