Bakterije roda Vibrio iz lubina (Dicentrarchus labrax Linnaeus, 1758) uzgojenih u istočnom Jadranu

master's thesis

• Title (english): Characterization of Vibrio species isolated from European sea bass (Dicentrarchus labrax Linnaeus, 1758) farmed on the east coast of the Adriatic sea
• Author: Tina Veić
• Mentor: Davor Zanella (mentor)
• Mentor: Snježana Zrnčić (mentor)
• Committee member: Davor Zanella (predsjednik povjerenstva)
• Committee member: Marija Gligora Udovič (član povjerenstva)
• Committee member: Domagoj Đikić (član povjerenstva)
• Committee member: Perica Mustafić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2016-04-13, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Zbog pravovremenog otkrivanja vibrioza uzgajanog lubina provedeno je istraživanje sa ciljem određivanja najvažnijih uzgojnih, morfoloških i biokemijskih karakteristika bakterija vrsta Vibrio anguillarum i Vibrio harveyi. Pri izradi diplomskog rada korištene su standardne bakteriološke metode, a za povrđivanje pripadnosti izolirane DNA bakterijama V. anguillarum i V. harveyi provedena je i molekularna identifikacija bakterija lančanom reakcijom polimeraze. Iz oboljelih riba izdvojeno je 6 sojeva V. anguillarum i 6 sojeva V. harveyi, te su njihove morfološke i biokemijske karakteristike međusobno uspoređene. Testirana je osjetljivost bakterija na pojedina antibakterijska svojstva sa ciljem liječenja i iskorjenjivanja vibrioze uzgajanog lubina. Dobiveni rezultati pokazuju da se vibrioza najčešće javlja u akutnom do subakutnom tijeku koji je karakteriziran krvarenjima oko usta, po osnovama peraja i perianalnoj regiji. Utvrđivanjem biokemijskih svojstava uočeno je da bakterijski sojevi V. harveyi za razliku od V. anguillarum nemaju sposobnost iskorištavanja galaktozidaze, razgradnje aminokiseline triptofan u indol, također nemaju sposobnost razgradnje želatine, te ne proizvode nitrate i acetoin. Dokazano je da su svi ispitivani sojevi osjetljivi do umjereno osjetljivi na niske koncentracije flumekvina, oksitetraciklina, oksolinske kiseline i trimetoprim-sulfametoksazola. Ovo istraživanje je pružilo osnovu za bržu dijagnostiku vibrioza i postavilo smjernice za uspješno i pravovremeno liječenje.

Abstract (english)

The aim of this study was to identify the most important growing, morphological and biochemical characteristics of two bacteria from the genus Vibrio; Vibrio anguillarum and Vibrio harveyi for early detection of vibriosis in farmed sea bass. During the studies carried out for this thesis we used standard bacteriological methods, but in order to confirm that isolated DNA belonged to bacteria V. anguillarum and V. harveyi we also performed a molecular identification using the polymerase chain reaction. From sick fish we isolated 6 strains of V. anguillarum and 6 strains of V. harveyi, and we compared their morphological and biochemical characteristics. To create the basis for successful treatment and prevention of vibriosis in farmed sea bass, we tested a sensitivity of each isolated bacteria to a certain antibacterial substance. Results showed that most often the disease appears in its acute or subacute course, characterised by hemorrhages around the mouth, on the fins basis and perianal region. Comparing biochemical properties of isolated bacterial strains of V. harveyi to V.anguillarum we concluded that the first ones do not have abillity to utilize galactosidase, to decompose tryptophan into indole, have no mechanism of degradation of gelatine and do not produce nitrates and acetoin. It is proved that all the strains are sensitive or moderately sensitive to low concentrations of flumequine, oxytetracycline, oxolinic acid and trimethoprim-sulfamethoxazole. This study provided basis for faster diagnosis and successful treatment of vibriosis.

• Keywords: lubin; vibrioza
• Keywords (english): sea bass; vibriosis
• Language: croatian
• URN:NBN: urn:nbn:hr:217:740993
• Study programme: Title: Ecology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra ekologije i zaštite prirode (magistar/magistra ekologije i zaštite prirode)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-01-25 13:46:31