Učinci fluorescentnih derivata indola na regulaciju staničnog ciklusa i preživljavanje stanica adenokarcinoma i fibroblasta pluća čovjeka

master's thesis

• Title (english): Effects of fluorescent indole derivates on the cell cycle regulation and survival of human adenocarcinoma cells and lung fibroblast cells
• Author: Jelena Puzić
• Mentor: Dragomira Majhen (mentor)
• Mentor: Nada Oršolić (komentor)
• Committee member: Nada Oršolić (predsjednik povjerenstva)
• Committee member: Biljana Balen (član povjerenstva)
• Committee member: Nenad Malenica (član povjerenstva)
• Committee member: Tomislav Ivanković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2025-01-29, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Fluorescentni indolni kationi zanimljivi su za terapeutsku upotrebu jer pokazuju jaku fluorescenciju i lokalizaciju u mitohondrijima. Modifikacijom indolnog prstena ovih spojeva, moguće je poboljšati njihova svojstva, poput fotostabilnosti i protutumorske aktivnosti, čime se povećava njihova učinkovitost u terapijama. Cilj ovog diplomskog rada bio je ispitati učinak novo sintetiziranih derivata indola na bazi vinil piridina na stanični ciklus, preživljenje, ekspresiju proteina uključenih u preživljavanje i regulaciju staničnog ciklusa te ispitati sposobnost lokalizacije spojeva u mitohondrijima stanica adenokarcinoma (A549) i fibroblasta pluća čovjeka (WI-38). Tretman stanica A549 i WI-38 spojevima FB404, FB411, FB414 i FB417 uzrokuje smanjenje preživljenja stanica. Smanjeno preživljenje stanica nakon tretmana spojevima nije uzrokovano aktivacijom apoptoze. Nadalje, spojevi FB404, FB406, FB411, FB414 i FB417 pokazuju značajnu lokalizaciju u mitohondrijima stanica A549 i WI-38. Tretman stanica A549 spojevima FB404, FB411, FB414 i FB417 uzrokuje zastoj u G0/G1 fazi staničnog ciklusa koji je vjerojatno posljedica povećane ekspresije proteina p21.

Abstract (english)

Fluorescent indole cations are of great interest for therapeutic use because they show strong fluorescence and localization in mitochondria. By modifying the indole ring of these compounds, it is possible to improve their properties, such as photostability and antitumor activity and therefore increase their effectiveness in therapies. The aim of this thesis was to examine the effect of newly synthesized indole derivatives based on vinyl pyridine on the cell cycle, survival, expression of proteins involved in survival and regulation of the cell cycle, and to examine the ability of the compounds to localize in the mitochondria of adenocarcinoma cells (A549) and human lung fibroblasts (WI-38). Treatment of A549 and WI-38 cells with compounds FB404, FB411, FB414 and FB417 causes a decrease in cell survival. Decreased cell survival after compound treatment was not caused by activation of apoptosis. Furthermore, the compounds FB404, FB406, FB411, FB414 and FB417 show significant localization in the mitochondria of A549 and WI-38 cells. Treatment of A549 cells with compounds FB404, FB411, FB414 and FB417 causes arrest in the G0/G1 phase of the cell cycle, which is probably due to increased expression of the p21 protein.

• Keywords: fluorescentni derivati indola; stanični ciklus; apoptoza
• Keywords (english): fluorescent indole derivatives; cell cycle; apoptosis
• Language: croatian
• URN:NBN: urn:nbn:hr:217:715724
• Study programme: Title: Experimental Biology-University graduate programme; Study programme type: university; Study level: graduate; Academic / professional title: sveučilišni/a magistar/magistra eksperimentalne biologije (sveučilišni/a magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2025-02-06 14:03:14