Utjecaj prekomjerne ekspresije akvaporina 3 na akvaporin 5, akvaporin 11 te glicerol kinazu u staničnoj liniji MCF7

master's thesis

• Title (english): Effects of aquaporin 3 overexpression on aquaporin 5, aquaporin 11, and glycerol kinase in MCF7 cell line
• Author: Paola Pahek
• Mentor: Ana Čipak Gašparović (mentor)
• Mentor: Nada Oršolić (komentor)
• Committee member: Nada Oršolić (predsjednik povjerenstva)
• Committee member: Dubravka Hranilović (član povjerenstva)
• Committee member: Maja Matulić (član povjerenstva)
• Committee member: Romana Gračan (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2024-10-16, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Akvaporin 3 (AQP3), akvagliceroporin i peroksiporin, transmembranski je protein odgovoran za transport glicerola, vodikovog peroksida i vode. Njegova prekomjerna ekspresija povezana je s progresijom tumora i njegovom malignošću, te se stoga proučava kao potencijalna terapijska meta i biomarker u istraživanju raka. S obzirom da AQP3 olakšava transport glicerola, cilj ovog rada bio je istražiti učinke prekomjerne ekspresije AQP3 i tretmana glicerolom na akvaporin 3 (AQP3), akvaporin 5 (AQP5), akvaporin 11 (AQP11) kao i na glicerol kinazu (GK), enzim koji regulira metabolizam glicerola. Rezultati su pokazali da prekomjerna ekspresija AQP3 ne utječe na ekspresiju AQP5 i GK. Tretman glicerolom ne utječe na AQP5 i GK, ali utječe na ekspresiju gena AQP3 i AQP11. Ekspresija AQP3 se povećala dok se ekspresija AQP11 smanjila nakon tretmana glicerolom.

Abstract (english)

Aquaporin 3 (AQP3), an aquaglyceroporin and peroxiporin, is a transmembrane protein responsible for a transport of glycerol, hydrogen peroxide and water. Its overexpression is linked to tumor progression and malignancy and is therefore studied as a potential therapeutic target and biomarker in cancer research. As aquaporin 3 facilitates transport of glycerol, the aim this thesis was to investigate the effects of AQP3 overexpression and glycerol treatment on aquaporin 3 (AQP3), aquaporin 5 (AQP5), aquaporin 11 (AQP11) as well glycerol kinase (GK), the rate limiting enzyme of glycerol metabolism. The results showed that the AQP3 overexpression does not affect the expression of AQP5, and GK. Glycerol treatment does not affect AQP5 and GK but affects AQP3 and AQP11 gene expression. AQP3 expression increased while AQP11 expression decreased after glycerol treatment.

• Keywords: akvaporini; AQP3; AQP11; glicerol; rak dojke
• Keywords (english): aquaporins; AQP3; AQP11; glycerol; breast cancer
• Language: croatian
• URN:NBN: urn:nbn:hr:217:150896
• Project: Number: IP-2020-02-3617; Title: Određivanje uloga akvaporina 3 i 5 u razvoju rezistencije tumora dojke na oksidacijski stres; Title: Elucidating the role of aquaporins 3 and 5 in the development of breast cancer resistance to oxidative stress; Acronym: AquaBCaRe; Leader: Ana Čipak Gašparović; Jurisdiction: Croatia; Funder: Hrvatska zaklada za znanost; Funding stream: Research Projects
• Study programme: Title: Experimental Biology-University graduate programme; Study programme type: university; Study level: graduate; Academic / professional title: sveučilišni/a magistar/magistra eksperimentalne biologije (sveučilišni/a magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2025-02-12 08:34:03