Praćenje karakteristika matičnih stanica osteosarkoma čovjeka tijekom progresije bolesti

master's thesis

• Title (english): Monitoring the characteristics of osteosarcoma human stem cells during disease progression
• Author: Andrija Ivanišin
• Mentor: Inga Urlić (mentor)
• Committee member: Inga Urlić (predsjednik povjerenstva)
• Committee member: Nada Oršolić (član povjerenstva)
• Committee member: Maja Matulić (član povjerenstva)
• Committee member: Petar Kružić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2024-09-24, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Osteosarkom najčešći je primarni tumor kosti, a karakteriziraju ga maligne stanice mezenhimskog porijekla koje stvaraju osteoide. Stopa dugotrajne izliječenosti ovog tumora ponajviše ovisi o prisutnosti metastaza, prvenstveno plućnih i koštanih za vrijeme dijagnoze, koje su ujedno i glavni uzročnik smrti u ovoj bolesti. Sve više istraživanja ističe matične stanice raka, populaciju tumorskih stanica koje imaju sposobnost samoobnove i diferencijacije u sve ostale tumorske stanice kao jedan od glavnih uzročnika otpornosti na kemoterapeutike i povratka bolesti. Iz tih razloga u ovom istraživanju pratili smo promjene u metastatskom potencijalu, rezistenciji na kemoterapeutike i aktivnosti telomeraze u tumorskim matičnim stanicama tijekom progresije bolesti: za vrijeme dijagnoze, nakon prvog ciklusa kemoterapije te nakon recidiva bolesti. Utvrđen je pad u udijelu matičnih stanica raka nakon prvog ciklusa kemoterapije te njegov porast nakon recidiva bolesti što je u korelaciji s metastatskim potencijalom tumora. Nije zabilježena aktivnost telomeraze ni u jednoj fazi bolesti što upućuje na moguću aktivnost alternativnog mehanizma produljenja telomera, što bi trebalo biti ispitano u daljnjim istraživanjima. Osteosarkom je pokazao najmanju rezistenciju na kemoterapeutike neposredno nakon prvog ciklusa kemoterapije.

Abstract (english)

Osteosarcoma is the most common primary bone tumor, characterized by malignant cells of mesenchymal origin, which produce osteoid. The percentage of long-term cure rate mostly depends on the presence of metastases, primarily of lung and bone during diagnoses, which are also the main cause of death in this disease. More and more research highlights cancer stem cells, a population of tumor cells that can self-renew and differentiate into all other cancer cells as a main source of chemotherapy resistance and disease recurrence. For these reasons in this research, we monitored the change in metastatic potential, chemotherapy resistance and telomerase activity in cancer stem cells during disease progression: during diagnoses, after the first round of chemotherapy and after disease recurrence. We noticed a decrease in cancer stem cell rate after the first round of chemotherapy along with its rise after the disease recurrence, which correlates with metastatic potential of the tumor. Telomerase activity was not registered in either disease phase, which might point to the activity of the alternative mechanism of telomere elongation, which should be tested in further research. Osteosarcoma showed the least resistance to the chemotherapeutic agents immediatly after the first round of chemotherapy.

• Keywords: osteosarkom; matične stanice; metastaze; telomeraza; ALT; rezistencija
• Keywords (english): osteosarcoma; stem cells; metastases; telomerase; ALT; resistance
• Language: croatian
• URN:NBN: urn:nbn:hr:217:492251
• Project: Number: IP-2020-02-9559; Title: Razvoj metoda matematičkog modeliranja u biologiji i medicini; Title: Methods developing in mathematical modelling in biology and medicine; Acronym: MethMathModBioMed; Leader: Miljenko Huzak; Jurisdiction: Croatia; Funder: Hrvatska zaklada za znanost; Funding stream: Research Projects
• Study programme: Title: Experimental Biology-University graduate programme; Study programme type: university; Study level: graduate; Academic / professional title: sveučilišni/a magistar/magistra eksperimentalne biologije (sveučilišni/a magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2025-02-12 13:54:58