Varijabilnost virusa Epstein-Barr na temelju promotora EBER u malignim tumorima

master's thesis

• Title (english): Epstein-Barr virus variability based on EBER promoter in malignant tumors
• Author: Roberto Galov
• Mentor: Petra Korać (mentor)
• Committee member: Petra Korać (predsjednik povjerenstva)
• Committee member: Snježana Židovec Lepej (član povjerenstva)
• Committee member: Inga Urlić (član povjerenstva)
• Committee member: Domagoj Đikić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2024-09-30, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Virus Epstein-Barr (EBV, od eng. Epstein-Barr virus) ljudski je onkogeni virus koji se može detektirati u organizmu više od 90% ljudske populacije. U većini slučajeva asimptomatski je prisutan u organizmu do domaćinove smrti ali povezan je i s nastankom mnogih ne malignih i malignih bolesti kao što su Hodgkinov limfom, T-stanični angioimunoblastični limfom i karcinom nazofarinksa. Opisana su dva tipa replikacijskog ciklusa EBV-a: litički i latentni. Na temelju latentnih gena EBV se može podijeliti na podtipove i njihove varijante koje pokazuju različitu geografsku rasprostranjenost. Molekule EBER male su molekule RNA prisutne u svim fazama latencije te imaju razne uloge u nastanku i razvoju malignih bolesti. U ovom istraživanju analizirane su sekvencije promotora EBER ukupno 91 pacijenta s područja Republike Hrvatske koji boluju od malignih bolesti. Prvi puta je proučena varijabilnost EBV-a na temelju promotora gena EBER te je konstruiran algoritam za određivanje novoopisanih podtipova i varijanti EBV-a. Rezultati su pokazali različitu zastupljenost varijanti u analiziranoj populaciji kao i statistički značajnu povezanost novih varijanti virusa s pojedinim dijagnozama malignih bolesti. Novoopisana varijabilnost EBV-a otvara vrata novim istraživanjima u svrhu boljeg razumijevanja genetike i onkogenog potencijala ovog virusa i njegovih interakcija s domaćinom.

Abstract (english)

Epstein-Barr virus (EBV) is a human oncogenic virus which can be detected in the organism of over 90% of the human population. In most cases, it is asymptomatically present in the organism until host's death, but is also a known cause of various non-malignant and malignant diseases such as Hodgkin's lymphoma, T-cell angioimmunoblastic lymphoma and nasopharyngeal carcinoma. Two types of EBV replication cycle have been described: lytic and latent. Based on latent genes, EBV can be classified into subtypes and their variants which exhibit different geographical distribution. EBERs are small RNA molecules present in all stages of latency and play various roles in oncogenesis and tumor maintenance. In this study EBER promoter sequence was analyzed in 91 samples from Croatian patients with various malignant diseases. This study proposes EBV variability analysis based on EBER promoter and presents a new algorithm used to determine new EBV variants. The results showed unequal distribution of new variants within the analyzed population as well as statistically significant connection between new variants and specific diagnoses which opens the door to future studies with a goal of better understanding the genetics and the oncogenic potential of EBV.

• Keywords: EBV; algoritam; genska varijabilnost; cHL; T-AIL; NPC
• Keywords (english): EBV; algorithm; gene variability; cHL; T-AIL; NPC
• Language: croatian
• URN:NBN: urn:nbn:hr:217:594515
• Study programme: Title: Experimental Biology-University graduate programme; Study programme type: university; Study level: graduate; Academic / professional title: sveučilišni/a magistar/magistra eksperimentalne biologije (sveučilišni/a magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2025-02-13 14:41:45