Povezanost majčine pretilosti i gestacijskog dijabetesa s ekspresijom monoamin oksidaze A u posteljici

master's thesis

• Title (english): Association of maternal obesity and gestational diabetes with placental expression of monoamin oxidase A
• Author: Iva Cahunek
• Mentor: Jasminka Štefulj (mentor)
• Mentor: Dubravka Hranilović (komentor)
• Committee member: Dubravka Hranilović (predsjednik povjerenstva)
• Committee member: Vesna Benković (član povjerenstva)
• Committee member: Domagoj Đikić (član povjerenstva)
• Committee member: Nada Oršolić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2024-10-24, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Majčina pretilost u trudnoći i gestacijski dijabetes melitus (GDM) su rastući javno-zdravstveni problemi širom svijeta, povezani s brojnim negativnim zdravstvenim posljedicama za majku i plod. Posteljica je organ koji osigurava izmjenu tvari i plinova između majčinog i fetalnog krvotoka. Serotonin, biogeni monoamin koji obnaša ulogu neurotransmitera, hormona i paraktinog/autokrinog glasnika, sudjeluje u regulaciji razvoja i različitih funkcija posteljice te je važan i za pravilan razvoj... More fetalnog mozga. Ključni enzim u razgradnji serotonina je monoamin oksidaza (MAO). Izoforma A ovog enzima (MAOA) ima preferencijalni afinitet za serotonin u odnosu na druge monoamine i visoko je eksprimirana u posteljici. Cilj ovog rada je istražiti jesu li majčina pretilost i GDM povezani s promijenjenom razinom proteina MAOA u posteljici. U tu svrhu, metodom western blot, određene su razine proteina MAOA u uzorcma posteljica ženske (N=48) i muške (N=50) novorođenčadi čije majke su imale različiti metabolički status u trudnoći. Razine proteina MAOA u posteljici nisu se statistički značajno razlikovale između novorođenčadi majki s različitim metaboličkim statusom, neovisno o spolu novorođenčadi. Dobiveni rezultati pokazuju da GDM i debljina nisu povezani s promijenjenom ekspresijom proteina MAOA u posteljici. Less

Abstract (english)

Maternal obesity during pregnancy and GDM are growing public health problems worldwide, associated with a number of negative health consequences for the mother and fetus. The placenta is an organ that ensures the exchange of substances and gases between the maternal and fetal bloodstreams. Serotonin, a biogenic monoamine that acts as a neurotransmitter, hormone and paracrine/autocrine messenger, participates in the regulation of the development and various functions of the placenta and... More is also important for the proper development of the fetal brain. The key enzyme in the breakdown of serotonin is monoamine oxidase (MAO). Isoform A of this enzyme (MAOA) has a preferential affinity for serotonin over other monoamines and is highly expressed in the placenta. The aim of this study is to investigate whether maternal obesity and GDM are associated with altered MAOA protein levels in the placenta. For this purpose, using the western blot method, MAOA protein levels were determined in placental samples of female (N=48) and male (N=50) newborns whose mothers had different metabolic status during pregnancy. The MAOA protein levels in the placenta were not statistically significantly different between the newborns of mothers with different metabolic status, regardless of the sex of the newborn.The obtained results show that GDM and obesity are not associated with altered MAOA protein expression in the placenta. Less

• Keywords: MAOA; placenta; 5-hidroksitriptamin; GDM; pretilost; trudnoća
• Keywords (english): MAOA; placenta; 5-hydroxytryptamine; GDM; obesity
• Language: croatian
• URN:NBN: urn:nbn:hr:217:919802
• Project: Number: IP-2018-01-6547; Title: Utjecaj majčinog metaboličkog stanja na serotoninski sustav posteljice i neonatusa: od metilacije DNA do funkcije proteina; Title: Influence of maternal metabolic state on placental and neonatal serotonin system: from DNA methylation to protein function; Acronym: PLANS; Leader: Jasminka Štefulj; Jurisdiction: Croatia; Funder: Hrvatska zaklada za znanost; Funding stream: Research Projects
• Study programme: Title: Experimental Biology-University graduate programme; Study programme type: university; Study level: graduate; Academic / professional title: sveučilišni/a magistar/magistra eksperimentalne biologije (sveučilišni/a magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2025-02-13 14:50:44