cfDNA kao biomarker

undergraduate thesis

• Title (english): cfDNA as a biomarker
• Author: Luka Tandarić
• Mentor: Petra Korać (mentor)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2017-09-04, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Od otkrića cfDNA (od eng. cell-free DNA) do danas, razmatrane su i istraživane mnoge njene primjene u molekularnoj medicini: od dijagnoze i praćenja tumora te predviđanja ozdravljenja pomoću specifičnih biomarkera, do otkrivanja i predviđanja poteškoća u razvoju ploda putem neinvazivne prenatalne dijagnostike. Danas su poznati mnogi biomarkeri specifični za određene maligne tumore i pomoću nekih od njih moguće je doći do zaključaka o pojavnosti/otpornosti na liječenje/stadiju tumora, no još uvijek se ne zna dovoljno da bi takvo što u medicini postalo široko primjenjivano. U prenatalnoj dijagnostici, cfDNA se pokazao kao iznimno vrijedan alat pri otkrivanju genomskih abnormalnosti. Kako bi se u budućnosti sprječila zloupotreba ove tehnologije, potrebno je uspostaviti detaljni etički kodeks. Općenito, cfDNA porijeklom iz tumora/fetusa, kraća je od normalne/majčine cfDNA, što daleko olakšava pročišćavanje i analizu.

Abstract (english)

Since the discovery of cfDNA (cell-free DNA), many of its applications in molecular medicine have been considered and researched: from diagnosing and monitoring tumors, and predicting disease outcome using specific biomarkers, to the detection and prediction of aberrant fetus development using noninvasive prenatal diagnostics. Today, many biomarkers specific for certain tumors types have been discovered and some of them can be used to help the decision making process about the presence/treatment resistance/stage of a tumor. Still, there is an insufficient amount of knowledge about the subject, which will have to be thoroughly analysed before wide use in medical practice. cfDNA has become an immensely valuable tool in prenatal diagnostics. To prevent future misuse, establishment of a detailed ethical codex is vital. Generally, tumor/fetal cfDNA is shorter than normal/maternal cfDNA, which makes purification and analysis far easier.

• Keywords: cfDNA; biomarker
• Keywords (english): cfDNA; biomarker
• Language: croatian
• URN:NBN: urn:nbn:hr:217:749472
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) molekularne biologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2018-09-04
• Created on: 2017-09-29 12:36:54