Istraživanje utjecaja dinamike molekularnih motora pri osciliaciji jezgre

master's thesis

• Title (english): Research on the influence of the dynamics of molecular motors during nuclear oscillations
• Author: Natalija Karlović
• Mentor: Matko Glunčić (mentor)
• Committee member: Matko Glunčić (predsjednik povjerenstva)
• Committee member: Damir Veža (član povjerenstva)
• Committee member: Ana Sušac (član povjerenstva)
• Committee member: Nenad Pavin (član povjerenstva)
• Committee member: Vito Despoja (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Physics); Zagreb
• Defense date and country: 2017-09-29, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Physics

Abstract

Ključni dio svakog životnog ciklusa je reprodukcija. Uspješna izmjena genetskog materijala, odnosno miješanje i rekombiniranje kromosoma zahtijeva usklađeno kretanje jezgre. Ovaj fenomen uzrokuju molekularni motori koji se kreću oko jezgre naprijed-nazad uz pomoć mikrotubula u samoj stanici. Međutim, kako motori i mikrotubuli surađuju zajedno u stvaranju ovih gibanja velikih razmjera, ostaje misterija. U svrhu pronalaženja odgovora, provedena su istraživanja gibanja jezgre u stanicama kvasca. Ta gibanja su pokretana motornim proteinima koji vrše silu na mikrotubule. Uočeno je da se motorni proteini dinamički raspoređuju duž cijele stanice u asimetričan uzorak koji generira sile koje uzrokuju gibanje. Kombinirajući kvantitativno snimanje žive stanice i laserske ablacije s teorijskim modelom, nalazimo da se dinamička raspodjela molekulskih motora pojavljuje samo kao rezultat promjena u mehaničkom soju koju detektiraju proteinski motori. Odnosno, formiranje prostorno-vremenskog uzorka unutar stanice, može se pojaviti kao rezultat mehaničkih znakova [1] koji se razlikuju od konvencionalne molekularne signalizacije, kao i od samoorganizacije stanice temeljene na kombinaciji biokemijskih reakcija i difuzije.

Abstract (english)

A key aspect of life is sexual reproduction, which requires concerted movement. For successful mixing of the genetic material, molecular motors move the nucleus back and forth inside the cell. How motors work together to produce these large-scale movements, however, remains a mystery. To answer this question, we studied nuclear movement in fission yeast, which is driven by motor proteins pulling on microtubules. We show that motor proteins dynamically redistribute from one part of the cell to the other, generating asymmetric patterns of motors and, consequently, of forces that generate movement. By combining quantitative live cell imaging and laser ablation with a theoretical model, we find that this dynamic motor redistribution occurs purely as a result of changes in the mechanical strain sensed by the motor proteins. Our work therefore demonstrates that spatio-temporal pattern formation within a cell can occur as a result of mechanical cues [1], which differs from conventional molecular signaling, as well as from self-organization based on a combination of biochemical reactions and diffusion.

• Keywords: dinamika molekularnih motora; oscilacija jezgre
• Keywords (english): dynamics of molecular motors; nuclear oscillations
• Language: croatian
• URN:NBN: urn:nbn:hr:217:856406
• Study programme: Title: Physics; specializations in: Education; Course: Education; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra edukacije fizike (magistar/magistra edukacije fizike)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-10-13 12:09:30