Antitumorsko djelovanje biljke Camellia sinensis

undergraduate thesis

• Title (english): Antitumor activity of plant Camellia sinensis
• Author: Ivana Jurić
• Mentor: Inga Marijanović (mentor)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2017-09-25, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Camellia sinensis pripada biljnoj porodici Theaceae te postoji u obliku zimzelenog grmlja ili malih stabala čiji se listovi i pupoljci koriste za proizvodnju čaja.Ukoliko ih se promatra s obzirom na "fermentaciju", postoje tri glavne skupine čajeva: nefermentirani (zeleni i bijeli čaj), djelomično fermentirani (oolong čaj) i potpuno fermentirani (crni čaj). S obzirom na to da se Camellia sinensisubraja u ljekovito bilje, ova biljka čajevca, zbog svojih kemijskih spojeva,ima važnu ulogu u prevenciji i liječenju tumora. Polifenolzelenog čaj EGCGuspješno obustavlja proliferaciju linije tumorskih stanica dojkeMCF-7/BOS stimuliranu djelovanjem okolišnog estrogena PCB 102. TakoĎer je ispitivan potencijalni citotoksični i prooksidativni učinak ekstrakta zelenog čaja i dva glavna flavonoida, epigalokatehin galata(EGCG) i epikatehin galata (ECG), na tumorskestanične linije grkljana (HEp2) i njezinu križno-rezistentnu staničnu liniju CK2. EGCG i ekstrakt zelenog čaja povećavaju oštećenje DNA u staničnoj liniji CK2 tijekom kratkog izlaganja. Citotoksičnost EGCG i ECG se povećava s vremenom inkubacije, dok je ekstrakt zelenog čaja izazvao peroksidaciju lipida ustaničnoj liniji CK2. Ispitivan je i ekstrakt bijelog čaja koji inhibira proliferaciju stanica tumora debelog crijeva HT-29, a štiti normalne stanice 3T3-L1 od oštećenja DNA vodikovim peroksidom. U ovom radu se nastoje istaknuti mogući korisni ljekoviti učinci biljke Camellia sinensis u kemoprevenciji i kemoterapiji različitih tipova stanica raka.

Abstract (english)

Camellia sinensis is a species of evergreen shrub or small tree whose leaves and leaf buds are used to produce tea. Concerning to the level of “fermentation”, it can be categorized into three major types: not fermented (green and white tea), partially fermented (oolong tea) and completely fermented (black tea). Camellia sinensis is a medicinal herb that plays an important role in prevention and treatment of tumors. Green tea polyphenol EGCG, suppressed the proliferation-stimulating activity of the environmentalestrogen PCB 102 on MCF-7/BOS breast cancer cells. Potential cytotoxic and prooxidative effects of green tea extract and its two main flavonoid constituents epigallocatechin gallate (EGCG) and epicatechin gallate (ECG) were also observed when tasted on human laryngeal carcinoma cell line (HEp2) and it's crossresistant CK2 cell line. The results showed that EGCG and green tea extract increased the DNA damage in the CK2 cell line during short exposure. The cytotoxicity of EGCG and ECG increased with the time of incubation. Green tea extract induced lipid peroxidation in the CK2 cell line. White tea extract, that inhibits the proliferation of HT-29 colon tumor cells and protects normal 3T3-L1 cells from DNA damage by hydrogen peroxide, was also explored. This paper presents a brief overview of beneficial therapeutic effects of Camellia sinensis in chemoprevention and chemotherapy of different cancer cells types.

• Keywords: Camellia sinensis; ljekovito bilje; kemoterapija
• Keywords (english): Camellia sinensis; medicinal herbs; chemotherapy
• Language: croatian
• URN:NBN: urn:nbn:hr:217:567621
• Study programme: Title: Ecology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) biologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-10-20 11:47:42