Proteom kokosovog mlijeka

undergraduate thesis

• Title (english): The proteome of coconut milk
• Author: Silvija Lihtar
• Mentor: Biljana Balen (mentor)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2015, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Kokosovo mlijeko je slatka, mliječno bijela tekućina, dobivena iz „mesa“ zrelog kokosa – ploda kokosove palme (Cocos nucifera L.). Bogato je vitaminima, mineralima i dobrim mastima te stoga ima veoma široku primjenu. Česta je namirnica u mnogim tropskim kuhinjama, na zapadu se uglavnom koristi kao napitak, a u veganskoj kuhinji služi kao zamjena za kravlje mlijeko kod kuhanja. Osim u prehrani, ima primjenu i u kozmetici, a smatra se da ima i mnoge povoljne utjecaje na ljudsko zdravlje. Proteom koksovog mlijeka bio je opsežno mapiran putem snimanja na tri pH vrijedosti pomoću datoteka rekombiniranih peptidnih liganda (CPLL). Ukupno je identificirano 307 jedinstvenih genskih produkata: 170 ih je otkriveno primjenom metode CPLL na 3 vrijednosti pH, 107 je pronađeno u kontroli, odnosno netretiranom materijalu, a 30 proteina je bilo zajedničko u oba seta podataka. CPLL tretman više je nego udvostručio broj istraženih proteina u kokosovom mlijeku i time je istraživanje proteoma kokosova mlijeka doseglo jedinstvenu dubinu. Baza podataka za ovu biljku od ranije je sadržavala 123 proteina, a 6 od tih je navedeno u rezultatima ovog istraživanja. To znači da je većina otkrivenih proteina identificirana samo na temelju homologije sa sekvencama pohranjenim u glavnoj biljnoj bazi podataka. Dobiveni skup podataka može biti početna točka nutricionistima i znanstvenicima koji proučavalju ljekovite sastojke u hrani za enukleaciju proteina odgovornih za neke povoljne učinke na ljudsko zdravlje koji se pridaju kokosovom mlijeku.

Abstract (english)

Coconut milk is a sweet, milky white liquid derived from the meat of mature coconut - the fruit of the coconut palm (Cocos nucifera L.). It is rich in vitamins, minerals and good fats and therefore has a very wide application. It is a common ingredient in many tropical cuisines, in the west is mainly used as a beverage, and in vegan cuisine it is used as a substitute for cow's milk. Other than in nutrition, it is also used in cosmetics, and is thought to have many beneficial effects on human health. The proteome of coconut milk has been extensively mapped via capture with combinatorial peptide ligand libraries (CPLL) at three pH values. A grand total of 307 unique gene products could be listed, 200 discovered via CPLL capture, 137 detected in the control, untreated material and 30 species in common between the two sets of data. CPLL treatment has more than doubled the total discovery of proteins in coconut milk, and with that the exploration of the coconut milk proteome has reached unprecednted depth. The database of this plant contained only 123 proteins, and 6 of those are listed in results of this exploration. It means that the vast majority of the classified proteins has been identified only by homologies with sequences deposited in the general Viridiplantae database. This unique set of data could be the starting point for nutricionists and researchers involved in nutraceutics for enucleating proteins responsible for some of the beneficial health effects attributed to coconut milk.

• Keywords: kokosovo mlijeko; proteom koksovog mlijeka
• Keywords (english): coconut milk; the proteome of coconut milk
• Language: croatian
• URN:NBN: urn:nbn:hr:217:458038
• Study programme: Title: Ecology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) biologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-12-15 11:02:40