Upotreba stres proteina u biomonitoringu

undergraduate thesis

• Title (english): Application of stress-proteins in biomonitoring
• Author: Mateja Nemet
• Mentor: Goran Klobučar (mentor)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2015, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

U zadnje vrijeme je izražena potreba za učestalijim nadzorom okoliša. U svrhu biomonitoringa traže se najpogodniji biomarkeri. Jedni od njih su proteini toplinskog stresa (eng. Heat shock proteini HSP). Hsp posebice porodica Hsp70 su sveprisutni i evolucijski očuvani proteini u skoro svim organizmima te se njihovi geni lako izoliraju i identificiraju u genomu. Njihova povećana ekspresija označava prisutnost denaturiranih proteina čija je konformacija promijenjena uslijed stresa te se je to dugi period pripisivalo onečišćenju. Danas se rade istraživanja koja pokazuju da povećana ekspresija Hsp70 proteina ne mora nužno značiti prisutstvo toksične tvari te da je potrebno bolje razumijevanje kompleksnosti Hsp odgovora na stres, a ujedno i bolje poznavanje fiziologije izabranog modelnog organizma.

Abstract (english)

Recently there has been a need for more frequent supervision of the environment. For the purpose of biomonitoring there is a constant search for the most suitable biomarkers. One of those are Heat shock proteins (HSP). HSP, especially Hsp70 family are ubiquitous proteins that are evolutionarily conserved in almost all organisms, their genes are easy to isolate and identify in the genome. They react to non-functional proteins with increased up-regulation. For a long period that was associated with cellular stress response due to environmental pollution. Today, researches have showed that up-regulation of Hsp70 proteins does not necessarily have to be triggered by the presence of toxic substances and that there is a need for better understanding of complexity of the HSP response to stress, and moreover for better understanding of the physiology of the selected model organism.

• Keywords: biomonitoring; proteini toplinskog stresa; HSP
• Keywords (english): biomonitoring; Heat shock proteins; HSP
• Language: croatian
• URN:NBN: urn:nbn:hr:217:975948
• Study programme: Title: Ecology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) biologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-12-15 12:07:17