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undergraduate thesis
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The role of hair in forensic analysis
Karlo Skube (2015)
University of Zagreb
Faculty of Science
Department of Biology
Metadata
TitleUloga dlaka u forenzičkoj analizi
AuthorKarlo Skube
Mentor(s)Inga Marijanović (thesis advisor)
Abstract
Dlaka je jedan od najčešćih dokaza korištenih u forenzičkoj analizi. Zbog jednostavnog prenošenja, lako može povezati sumnjivca s mjestom zločina ili žrtvom. Usporedni mikroskop koristi se pri mikroskopskoj usporedbi poznatog i istraživanog uzorka dlake. Mikroskopska analiza dlake može pomoći u određivanju rase, područja rasta dlake, dobi i spola. Ukoliko iščupana dlaka ima sačuvan korijen ili je na sebi zadržala okolnog folikularnog tkiva, iz nje se može izolirati nuklearna DNA, napraviti DNA profil i individualizirati osobu. Također, pomoć pri identifikaciji pruža i mitohondrijska DNA, ali ona ne može služiti u individualizaciji jer se nasljeđuje po majčinoj liniji. Poboljšavanjem postojećih metoda jednostavnim unaprijeđenjima kao što je fiksacija dlake u apsolutnom etanolu te razvitkom novih metoda značajno se povećava uspješnost izolacije DNA. Upotrebom drugačijih boja kao što je Harris’s Haematoxylin dolazi do poboljšanja dvije stvari. Prva je smanjenje sigurnosnih rizika jer je Harris’s Haematoxylin mnogo sigurnija boja od DAPI-ja. Drugo poboljšanje je eliminacija potrebe za korištenjem fluorescencijskog mikroskopa jer je DNA vidljiva i bez poticanja fluorescencije određenim valnim duljinama.
Keywordshair hair sample forensic analysis
Parallel title (English)The role of hair in forensic analysis
GranterUniversity of Zagreb
Faculty of Science
Lower level organizational unitsDepartment of Biology
PlaceZagreb
StateCroatia
Scientific field, discipline, subdisciplineNATURAL SCIENCES
Biology
Study programme typeuniversity
Study levelundergraduate
Study programmeMolecular Biology
Academic title abbreviationuniv. bacc. biol. mol.
Genreundergraduate thesis
Language Croatian
Defense date2015
Parallel abstract (English)
Hair is one of the most common evidence used in forensic analysis. Due to a simple transfer, it can easily connect a suspect to a crime scene or victim. Comparison microscope is used in microscopic comparison of known and unknown hair sample. Microscopic analysis of the hair can help determine someone’s race, part of the body where hair was growing, age and gender. If it has preserved root or parts of surrounding follicular tissue, it can be used for nuclear DNA isolation, profiling and individualization of a person. Mitochondrial DNA can also help in identification but it cannot individualize a person because it is inherited maternally. Improvement in existing methods with simple advancements such as fixation of hair in absolute ethanol and development of new methods significantly increases efficiency of DNA isolation. Using different colors such as Harris’s Haematoxylin, improvement occurs in two things. The first is to reduce security risks because Harris’s Haematoxylin is much more safer than DAPI. Second improvement is the elimination of the need to use fluorescent microscope because DNA is visible without simulating fluorescence by certain wavelengths.
Parallel keywords (Croatian)dlaka uzorak dlake forenzička analiza
Resource typetext
Access conditionOpen access
Terms of usehttp://rightsstatements.org/vocab/InC/1.0/
URN:NBNhttps://urn.nsk.hr/urn:nbn:hr:217:080422