Experimental methods in functional genomics

undergraduate thesis

• Author: Ivan Mikičić
• Mentor: Kristian Vlahoviček (mentor)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2015, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Over the past few years, massively parallel DNA sequencing platforms have become widely available, dramatically reducing the cost of DNA sequencing. The fast and low-cost sequencing approaches not only change the landscape of genome sequencing projects but also usher in new opportunities for sequencing in various applications, among other in functional genomics. The aim of functional genomics studies is to understand the complex relationship between genotype and phenotype on a genome-wide scale. Studies investigate a range of processes such as transcription, translation and epigenetic regulation, in an attempt to answer relevant biological questions. Newly developed techniques, such as RNA-seq, ChIP-seq and 3C-based methods found their use in some of the most massive projects, such as the ENCODE project. The aim of this review was to introduce some of the most common techniques in functional genomics and to explain their possibilities, advances, applications and challenges.

• Keywords: experimental methods; functional genomics
• Language: english
• URN:NBN: urn:nbn:hr:217:648584
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) molekularne biologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-12-21 12:29:03