Bioakumulacija metala u tragovima u dagnjama (Mytilus galloprovincialis) iz Malostonskog zaljeva

doctoral thesis

• Title (english): Bioaccumulation of trace metals in mussel (Mytilus galloprovincialis) from Mali Ston Bay
• Author: Nenad Vuletić; MBZ: 313303
• Mentor: Ivana Ujević (mentor); MBZ: 171094
• Mentor: Grozdan Kušpilić (komentor); MBZ: 135454
• Committee member: Dubravka Matković-Čalogović (predsjednik povjerenstva)
• Committee member: Nives Galić (član povjerenstva)
• Committee member: Ivana Ujević (član povjerenstva); MBZ: 171094
• Granter: University of Zagreb; Faculty of Science; (Department of Chemistry); Zagreb
• Defense date and country: 2015-07-15, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry
• Universal decimal classification (UDC): 54 - Chemistry. Crystallography. Mineralogy

Abstract

Određivanje metala u tragovima, morskih biotoksina i drugih opasnih tvari u jestivom tkivu školjkaša važno je za procjenu ekološkog statusa okoliša u kojem žive i zdravstvene ispravnosti školjkaša u cilju zaštite zdravlja ljudi. Cilj ovog rada bio je utvrđivanje pozitivne ili negativne korelacije sadržaja metala u tragovima u tkivima dagnji s pozitivnim rezultatima bioloških testova za određivanje prisustva toksina koji uzrokuju dijaretičko trovanje školjkašima (DSP). U ovom radu izmjereni su maseni udjeli metala u tragovima (Cd, Pb, Zn, Cu, Cr i Ni) koji su se bioakumulirali u tkivu dagnje (Mytilus galloprovincialis). Dagnje su prikupljene u periodu od siječnja do lipnja 2011. godine na uzgajalištima u akvatoriju južnog dijela Jadrana. U tom je periodu prilikom provođenja nacionalnog monitoring programa morskih biotoksina, primjenom biološkog testa, ustanovljena prisutnost DSP toksina u isptivanim dagnjama. Pojava toksičnosti započela je u siječnju 2011. i trajala je do sredine ljeta, s najvećim brojem DSP pozitivnih uzoraka u lipnju. Razlog za pozitivan biološki test mogu biti i druge manje toksične tvari ili tvari koje nisu toksične (cink ili masne kiseline). Dagnja je organizam koji kroz prostornu i vremensku raspodjelu pokazuje promjene u masenim udjelima metala u tragovima zbog čega se često koristi u biomonitoringu.

Abstract (english)

The contamination of shellfish with marine biotoxins, trace metals or other contaminants is an ecological, food safety and a human health concern. The goal of the present study was determination of a positive or negative correlation of the content of trace metals (Cd, Pb, Zn, Cu, Cr and Ni) with a positive or negative diarrhetic shellfish poisoning (DSP) bioassay in shellfish tissue. The concentrations of trace metals that were bioaccumulated in the soft tissue of mussel (Mytilus galloprovincialis) were measured in this study. Mussels were collected on breeding farms in the Mali Ston Bay from seven sites from January till June 2011. In that period toxicity was recorded in the course of the official analyses of marine biotoxins under the National Monitoring Program. According to the official method for DSP toxins, the mouse bioassay test, positive results for DSP toxins were reported. In some areas, the occurrence of toxicity started in January 2011 and lasted until mid-summer, with a maximum number of DSP positive samples in June. However, other less toxic or non-toxic substances (zinc or fatty acids) can be the cause for a positive bioassay. Mussel is an organism which is able to show spatial and temporal changes in trace metals concentrations and is a suitable candidate to be used in biomonitoring surveys.

• Keywords: bioakumulacija; dagnja; DSP toksini; metali u tragovima; prostorna raspodjela; vremenska raspodjela
• Keywords (english): bioaccumulation; diarrhetic shellfish poison toxins; mussel; spatial distribution; temporal distribution; trace metals
• Language: croatian
• URN:NBN: urn:nbn:hr:217:568589
• Study programme: Title: Doctoral study in chemistry; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje kemija (doktor/doktorica znanosti, područje prirodnih znanosti, polje kemija)
• Type of resource: Text
• Extent: 126 str. ; 30 cm
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-02-14 13:40:46