Posttranskripcijsko procesiranje primarnog transkripta za tRNA

undergraduate thesis

• Title (english): Post-transcriptional processing of tRNA-precursor molecule
• Author: Ivana Čavka
• Mentor: Jasmina Rokov Plavec (mentor)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2016, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Glavna tema ovog rada je procesiranje primarnog transkripta za tRNA. Molekule tRNA u stanici imaju veoma važne uloge. Osim njihove primarne uloge u translaciji, molekule tRNA sudjeluju i u staničnoj regulaciji i odgovoru stanice na stres. Funkcionalnost biomolekula je osigurana ispravnošću njihove konformacije, koja se stječe kroz različite procese sazrijevanja molekule. Molekule tRNA nisu iznimka od ovog pravila, pošto je ispravno obavljanje njihovih uloga od iznimne važnosti za normalno funkcioniranje stanice. Primarni transkripti uvelike se razlikuju od zrele, funkcionalne molekule tRNA i moraju proći kroz različite oblike posttranskripcijskog procesiranja kako bi sazreli. Ovo procesiranje kod većine organizama uključuje procesiranje krajeva, izrezivanje introna i uvođenje različitih modifikacija ribonukleotida. Rad nudi kratak uvod u svaki od ovih koraka uz navedene najbolje poznate primjere iz tri domene (Archaea, Bacteria, Eukaryota), a uz glavnu temu obrađene su i one usko povezane uz procesiranje poput raznolikosti gena za tRNA i putovanja molekula tRNA kroz eukariotsku stanicu tijekom biogeneze.

Abstract (english)

Main topic of this paper is post-transcriptional processing of tRNA precursor molecule. In a cell, tRNA molecules play a very important role. Except their well-known primary role in translation, tRNA molecules are also involved in processes such as stress response and different cellular regulation pathways. In order to properly play their roles in the cell, biomolecules have to be folded correctly. tRNAs are not exceptions to this rule, since maintenance of processes that involve tRNA molecules is crucial for cell’s viability. tRNA precursor molecules differ substantially from the mature tRNAs and, in order to become functional molecules, they have to undergo post-transcriptional processing. Processing mainly involves end processing, splicing, and ribonucleotide modifications. Paper offers a short introduction to each step with best known examples from all three domains of life (Archaea, Bacteria, Eukaryota). Except for the post-transcriptional processing, some closely related topics, such as diversity of tRNA genes and tRNA subcellular trafficking in eukaryotic cells, are also discussed.

• Keywords: molekule tRNA; posttranskripcijsko procesiranje
• Keywords (english): tRNA molecules; post-transcriptional processing
• Language: croatian
• URN:NBN: urn:nbn:hr:217:408751
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) molekularne biologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-01-02 08:24:52