Stanične linije u istraživanju malignih tumora

undergraduate thesis

• Title (english): Cell lines in cancer research
• Author: Valentina Hribljan
• Mentor: Petra Korać (mentor)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2016, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Stanične linije malignih tumora najkorišteniji su model u istraživanjima tumorogeneze. Njihova prednost je lako rukovanje, dostupnost i cijena, ali imaju nedostatak da se kod njih, za razliku od istraživanja na animalnim modelima ne može pratiti progresija tumora. Za potpuno razumijevanje tumora potrebno je zato kombinirati rezultate dobivene uz pomoć različitih modela. Kao biološki entiteti, stanične linije malignih tumora podložne su prirodnoj selekciji. Uvjeti u kojima se uzgajaju znatno utječu na njihov genotip, a time i na fenotip te njihovu reprezentativnost kao modela. Postoji preko 1000 različitih staničnih linija kojima se nastoji obuhvatiti raznolikost tumora. One su glavni pretklinički model za testiranje djelovanja kemoterapeutika, a njihova raznolikost omogućava personalizirani pristup liječenju. Neke stanične linije korištenije su od drugih, ali nisu nužno reprezentativniji model od onih koje se rjeđe koriste. Enciklopedija staničnih linija malignih tumora i Atlas genoma malignih tumora omogućavaju usporedbu staničnih linija i tumora in vivo na genetičkoj razini te odabir reprezentativnih staničnih linija. Stanične linije malignih tumora koriste se i u druge svrhe kao što je npr. testiranje toksičnosti neke tvari, istraživanja virusa i bakterija itd. Neke stanične linije mogu poslužiti kao in vitro modeli za istraživanje tumorskih matičnih stanica– novih potencijalnih meta za kemoterapeutike.

Abstract (english)

Cancer cell lines are the most widely used models in cancer research. They are fast-growing cells, easy to handle compared to other cancer models and there is huge variety of different kinds in cell line banks. Other models have their advantages, e.g. animal models can be used for studying cancer progression. Therefore, to understand the complete picture of tumorogenesis it is the best to combine different models. Conditions in cell culture determine the genotype and therefore also phenotype of cells so it is important to optimise cell culture conditions in order to get cancer cell lines that will be representative models in cancer research. They are main models for preclinical drug testing - over 1000 different cell lines represent cancer diversity and enable personalized treatment approach. The most widely used cell lines are not necessary the best models. The Cancer Genome Atlas and Cancer Cell Line Encyclopedia enable comparison of genomic profiles of cancer cell lines and tumors in vivo in order to find cell lines that are the most relevant for certain research. Cancer cell lines can be used in other researches such as testing toxicity of some agents. Some cancer cell lines can be used as in vitro model for cancer stem cell research – model for new potential targets of drug treatment.

• Keywords: stanične linije malignih tumora
• Keywords (english): cancer cell lines
• Language: croatian
• URN:NBN: urn:nbn:hr:217:630031
• Study programme: Title: Ecology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) biologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-01-02 10:44:58