The role of brassinosteroids and salicylic acid in plant defense response to Potato spindle tuber viroid infection

doctoral thesis

• Title (croatian): Uloga brasinosteroida i salicilne kiseline u obrambenom odgovoru biljaka na zarazu viroidom vretenastoga gomolja krumpira
• Author: Jasna Milanović
• Mentor: Snježana Mihaljević (mentor); MBZ: 189640
• Committee member: Željka Vidaković Cifrek (predsjednik povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2018, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology
• Universal decimal classification (UDC): 578/579 - Virology. Microbiology

Abstract

Viroids are plant–pathogenic molecules characterized by a single–stranded circular non–coding RNA
genome. Despite their genomic simplicity, these plant pathogens are able to induce disease in a wide
range of host plants with significant economic impact. The surveillance activities described in this
study, revealed the presence of Potato spindle tuber viroid (PSTVd) in Croatia in two ornamental
species, while no PSTVd infection was found in tomato or potato. This study was also aimed to
evaluate the effects of PSTVd infection on endogenous plant growth regulator (PGR) content, and to
identify common, but also specific, features of systemic hormonal response in solanaceous plant
species in which PSTVd causes different degrees of symptoms. Endogenous brassinosteroide
castasterone, salicylic acid, abscisic acid, indole–3–acetic acid, jasmonic acid and its precursor
cis–12–oxophytodienoic acid were quantified using UHPLC–MS/MS. The study provides new
evidences about the involvement of different PGRs and antioxidant enzymes in plant defense
response against PSTVd infection, in both symptomatic and asymptomatic solanaceous species.

Abstract (croatian)

Viroidi su najmanji poznati uzročnici bolesti biljaka čiji genom karakterizira nekodirajuća
jednolančana kružna molekula RNA. Unatoč njihovoj jednostavnosti, ovi biljni patogeni uzrokuju
bolesti mnogih kultiviranih biljaka širom svijeta, kao i značajne ekonomske gubitke. Bolest
uzrokovana viroidom vretenastoga gomolja krumpira (Potato spindle tuber viroid, PSTVd),
otkrivena je u okviru redovitih nadzornih aktivnosti u dvije ukrasne vrste u Hrvatskoj, dok zaraza u
rajčici i krumpiru nije potvrđena. Cilj ovog istraživanja ujedno je bio ispitati utjecaj zaraze viroidom
vretenastoga gomolja krumpira na biljne regulatore rasta te odrediti zajedničke, kao i vrsno
specifične značajke hormonskog odgovora u vrstama iz porodice Solanaceae koje pokazuju različite
simptome bolesti. Izmjeren je sadržaj endogenog brasinosteroida kastasterona, salicilne kiseline,
apscizinske kiseline, indol–3–octene kiseline, jasmonske kiseline i cis–12–oksofitodienoične
kiseline, primjenom UHPLC–MS/MS metode. Ova studija daje nove dokaze o ulozi biljnih
regulatora rasta i antioksidacijskih enzima uslijed staničnog odgovora biljke na zarazu viroidom
PSTVd

• Keywords: PSTVd; Solanaceae; plant growth regulators; antioxidant response; latent infection
• Keywords (croatian): PSTVd; Solanaceae; biljni regulatori rasta; antioksidacijski odgovor; latentna zaraza
• Language: english
• URN:NBN: urn:nbn:hr:217:877399
• Study programme: Title: Biology; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2019-12-23
• Created on: 2018-01-22 10:08:18