Učinak arbutina na oksidacijski stres i na primarna oštećenja DNA u štakora

master's thesis

• Title (english): Effect of arbutin on oxidative stress and on primary DNA damages in rats
• Author: Iva Đelatić
• Mentor: Vesna Benković (mentor)
• Committee member: Vesna Benković (predsjednik povjerenstva)
• Committee member: Božena Mitić (član povjerenstva)
• Committee member: Zlatko Mihaljević (član povjerenstva)
• Committee member: Duje Lisičić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2017-12-14, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Fenolni glikozid arbutin sekundarni je metabolit u mnogim biljkama, no također se može proizvesti kemijskom sintezom. Iznimno je učinkovit kod liječenja urinoinfekcija, te ima antioksidacijska, antiupalna, antitumorska i antibakterijska svojstva. Povećana prisutnost slobodnih kisikovih i dušikovih vrsta u organizmu dovodi do ozljeda stanica te pojave mnogih bolesti. Iz tog razloga provodi se niz istraživanja s ciljem pronalaženja tvari koje bi svojim djelovanjem mogle štititi stanice, organe i ljudski organizam u cijelosti. Cilj ovog istraživanja je ispitati učinak arbutina na stanice jetre, bubrega i mozga štakora soja Lewis oba spola tretiranih arbutinom u koncentraciji od 200 mg/kg per oralno tijekom 28 dana. Određivanjem aktivnosti katalaze i superoksid dismutaze te koncentracije MDA odredit će učinak arbutina na pojavu oksidacijskog stresa. Za procjenu primarnih oštećenja DNA u stanicama koristit će se standardna izvedba komet testa u alkalnim uvjetima. Rezultati su pokazali kako arbutin ne utječe na intenzitet lipidne peroksidacije. Superoksid dismutaza pokazuje pad aktivnosti u bubregu ženke štakora, dok je katalazna aktivnost snižena kod stanica mozga, također kod ženki. Komet test je pokazao povišene vrijednosti DNA oštećenja u jetri, a snižene u mozgu štakora. No, generalno se može zaključiti kako arbutin nije toksično djelovao na stanice bubrega jetre i mozga štakora.

Abstract (english)

Phenolic glycoside arbutin is a secondary metabolite in many plants and it can also be produced by chemical synthesis. It is extremely effective in the treatment of urinary infections and has antioxidative, anti-inflammatory, anti-tumor and antibacterial properties. Increased presence of free oxygen and nitrogen species in the organism leads to cell injuries and the appearance of many diseases. For this reason, many researches are being conducted, with a goal to find substances that could protect cells, organs and the human body in their entirety. The aim of this research is to examine the antioxidant effect of arbutin on liver, kidney and brain of rats (Lewis strain) of both sexes treated orally with arbutin at a concentration of 200 mg/kg for 28 days. Determination of catalase and superoxide dismutase activity and MDA concentration will determine if arbutin acts on oxidative stress. To assess primary DNA damage in cells, a standard comet test will be used in alkaline conditions. The results showed that arbutin does not affect the intensity of lipid peroxidation. Superoxide dismutase shows a fall in activity in the rat female kidney, while the catalytic activity is reduced to the brain cells, also in females. The comet test showed elevated values in the liver and lowered in the brain of the rat. However, it can generally be concluded that arbutin did not affect toxicity of the liver and brain kidney cells.

• Keywords: oksidacijski stres; ROS; lipidna peroksidacija; arbutin; primarna oštećenja; komet test
• Keywords (english): oxidative stress; ROS; lipid peroxidation; arbutin; primary damages; kommet test
• Language: croatian
• URN:NBN: urn:nbn:hr:217:005540
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-01-31 09:12:51