Adhezija stanica fitoplanktona na nabijenoj međupovršini elektroda/vodena otopina elektrolita

master's thesis

• Title (english): Adhesion of phytoplankton cells at a charged electrde/aqueous electrolyte interface
• Author: Ivana Grubeša
• Mentor: Nadica Ivošević DeNardis (mentor)
• Committee member: Marko Močibob (predsjednik povjerenstva)
• Committee member: Davor Kovačević (član povjerenstva)
• Committee member: Iva Juranović Cindrić (član povjerenstva)
• Committee member: Morana Dulić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Chemistry); Zagreb
• Defense date and country: 2016-12-19, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry

Abstract

U ovom radu ispitan je rast i prilagodba morskog fitoplanktona Dunaliella tertiolecta u dva vodena medija različitog saliniteta primjenom elektrokemijske metode, i tankoslojne kromatografije. Elektrokemijska karakterizacija staničnih suspenzija ukazuje na slična adhezijska ponašanja stanica u širokom području potencijala na elektrodi za oba medija. Veća fiziološka aktivnost stanica u smislu izlučenih biomolekula i mikrometarskih čestica detektirana je u mediju nižeg saliniteta. Tankoslojnom kromatografijom dobiven je porast koncentracije ukupnih lipida s porastom gustoće stanica za oba medija. Veća koncentracija ukupnih lipida određena je u mediju nižeg saliniteta. Najzastupljeniji membranski lipidi su glikolipidi koji variraju sa starošću kulture kod višeg i nižeg saliniteta. Kombinacijom dviju metoda omogućuje se bolje razumijevanje ponašanja stanica u vodenom sustavu u uvjetima okolišnog stresa (npr. kod promjene saliniteta).

Abstract (english)

In this thesis growth and adaptation of marine phytoplankton Dunaliella tertiolecta was examined in two media of different salinity using electrochemical methods and thin layer chromatography. Electrochemical characterization of cell suspensions showed similar adhesion behavior of cells in the wide range of applied potentials at the electrode in the both media. Higher physiological activity of cells due to the presence of exudates of biomolecules and micrometer sized particles was detected in the lower salinity medium. Thin layer chromatography showed increase in concentration of the total lipids with the cell density, for the both media. Higher concentration of total lipids was found in the lower ionic strength. Determined predominant membrane lipids were glycolipids that varied with aging of the cell culture at lower and higher salinity. Combination of electrochemical methods and thin layer chromatography enables better understanding of behavior of phytoplankton cells in aquatic system under environmental stress condition (e.g. variation of salinity).

• Keywords: adhezija; D. tertiolecta; fitoplankton; lipidi; kronoamperometrija; kromatografija
• Keywords (english): adhesion; chromatography; chronoamperometry; D. tertiolecta; lipids; phytoplankton
• Language: croatian
• URN:NBN: urn:nbn:hr:217:846376
• Study programme: Title: Graduate Studies in Chemistry; specializations in: Research (scientific-professional) programme; Course: Research (scientific-professional) programme; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-01-26 09:16:51