Procjena genotoksičnog učinka karbofurana u miša primjenom alkalnog komet-testa

master's thesis

• Title (english): Genotoxic carbofuran effect evaluation in mice by alkaline comet assay ussage
• Author: Ana Bajić
• Mentor: Davor Želježić (mentor)
• Mentor: Vesna Benković (sumentor)
• Committee member: Vesna Benković (predsjednik povjerenstva)
• Committee member: Davor Želježić (član povjerenstva)
• Committee member: Mirjana Pavlica (član povjerenstva)
• Committee member: Renata Šoštarić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2009, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Cilj istraživanja bio je istražiti genotoksični učinak insekticida karbofurana (2, 3-dihidro-2,2- dimetilbenzofuran-7-il-metilkarbamata) i njegove formulacije Geocid ST 35 (350g/l) primjenom alkalnog komet testa na stanicama organa soja miševa Swiss albino u uvjetima in vivo. Procjena genotoksičnosti provedena je u stanicama bubrega, jetre, koštane srži i leukocitima primjenom dvaju
parametara komet testa: dužine repa i intenziteta repa. Karbofuranom i Geocidom ST 35, svakodnevno tijekom 14 dana, obrađivane su četiri skupine životinja s dozama aktivne tvari u vrijednostima ADI i 1/100LD50 i uspoređene s kontrolnom skupinom. Utvrđeno je znatno veće oštećenje DNA u stanicama svih organa skupina obrađenih formulacijom Geocidom ST 35 za vrijednosti ADI i 1/100LD50 u odnosu na stanice životinja obrađenih čistom aktivnom tvari karbofuran u istim dozama. I za aktivnu tvar i za formulaciju intenzitet oštećenja bio je proporcionalan dozi pesticida.U stanicama bubrega, jetre i koštane srži utvrđena su podjednaka oštećenja molecule DNA, dok je razina genomskim oštećenja u leukocitima bila nešto niža. Na temelju dobivenih rezultata može se zaključiti da karbofuran i formulacija Geocid ST 35 genotoksično djeluju na stanice svih ispitivanih organa miševa, ali ni karbofuran ni formulacija nisu pokazali specifičnost genotoksičnog učinka prema nijednom organu. Oštećenja utvrđena u stanicama jetre, bubrega i koštane srži, osim genotoksičnog djelovanja karbofurana rezultat su i fizioloških procesa u tim tkivima.

Abstract (english)

The aim of research was to explore the genotoxic impact of carbofouran pesticide (2,3-dihydro-2,2- dimethyl-7-benzofuranol methylcarbamate) and its formulation Geocid ST 35 (350g/l) by alkali comet assay usage on four organs´ cells of mice strain swiss albino in conditions in vivo. The genotoxic evaluation was made on kidney, liver, bone marrow cells and leukocytes using two comet assay parameters: tail length and tail intensity. During fourteen day period, four groups of animals were treated by carbofouran and Geocid ST 35 with an active substance doses in values of ADI i 1/100LD50 and were compared with the control group. Both active supstance and formulation have shown damage intensity proportional to pesticide doses. Kidney, liver and bone marrow cells have shown similar DNA damage while genom level was slightly lower in leukocytes. Based on given results it can be concluded that carbofuran and Geocid ST 35 have genotoxic effect on all treated murine organs, but niether carbofuran nor its formulation have shown specificy of genotoxic effect on niether of organs. Demage detected in all organ cells exept lukocytes is a result of both carbofuran genotoxity impact and physiological proceses in those tissues.

• Keywords: karbofuran; Geocid ST 35; komet-test; genotoksičnost; miš
• Keywords (english): carbofuran; Geocid ST 35; comet assay; genotoxicity; mice
• Language: croatian
• URN:NBN: urn:nbn:hr:217:564873
• Study programme: Title: Ecology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra ekologije i zaštite prirode (magistar/magistra ekologije i zaštite prirode)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-03-06 09:14:18