Genotoksičan učinak talijeva(I) acetata na meristemske stanice boba (Vicia faba L.) i luka (Allium cepa L.)

master's thesis

• Title (english): Genotoxic effect of thallium(I) acetate on meristematic cells of broad bean (Vicia faba L.) and onion (Allium cepa L.)
• Author: Anamarija Melnjak
• Mentor: Mirjana Pavlica (mentor)
• Committee member: Mirjana Pavlica (predsjednik povjerenstva)
• Committee member: Ines Radanović (član povjerenstva)
• Committee member: Dubravka Matković-Čalogović (član povjerenstva)
• Committee member: Davor Kovačević (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2009, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

U ovom radu je istraženo djelovanje talijeva(I) acetata na meristemske stanice luka i boba. Analizirana je mitotska aktivnost te vrsta i učestalost kromosomskih i mitotskih aberacija nakon 24 i 72 sata tretmana, kao i nakon 72 sata oporavka. Rezultati su pokazali da talijev(I) acetat inhibira rast i mitotsku aktivnost u meristemskim stanicama obiju vrsta. Neposredno nakon tretmana kao i perioda oporavka uočene su multipolarne anafaze, mostovi, zaostali kromosomi, slijepljeni kromosomi i formiranje mikronukleusa. Iako su u ovom istraživanju korištene dvije biljne vrste, običan luk Allium cepa pokazao se kao osjetljivija vrsta u procjeni genotoksičnosti talija u odnosu na bob.

Abstract (english)

In the present study the effects of thallium(I) acetate on the root meristem cells of Allium cepa and Vicia faba were examined. The analysis of mitotic activity as well as the type and frequency of chromosomal and mitotic aberrations were done after 24h and 72h treatment period, as well as after 72h recovery period. The result showed that thallium inhibited the root growth as well as mitotic activity in the meristematic cells of both species. Thallium treatment induced multipolar anaphases, bridges, laggards, stickiness and micronucleus formation which were also observed after 72h recovery period. Although both test system were used for genotoxicity assessment, Allium cepa was shown to be more sensitive plant model organism than Vicia faba.

• Keywords: Vicia faba L.; Allium cepa L.; Allium-test; talijev(I) acetat; genotoksičnost
• Keywords (english): Vicia faba L.; Allium cepa L.; Allium-test; thallium(I) acetate; genotoxicity
• Language: croatian
• URN:NBN: urn:nbn:hr:217:107754
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-03-13 13:43:13