Utjecaj okolišnih i genetičkih čimbenika na krvni tlak stanovništva otoka Visa

master's thesis

• Title (english): The effect of enviromental and genetic factors on blood pressure in the population of the island Vis
• Author: Tonka Roša
• Mentor: Nina Smolej-Narančić (mentor)
• Committee member: Mirjana Kalafatić (predsjednik povjerenstva)
• Committee member: Nina Smolej-Narančić (član povjerenstva)
• Committee member: Zdravko Dolenec (član povjerenstva)
• Committee member: Vesna Benković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2009, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

U ovom istraživanju nastojalo se utvrditi utječe li ACE I/D polimorfizam, kao primjer genetičkog čimbenika unutar jednog od gena odgovornog za regulaciju krvnog tlaka, na krvni tlak čovjeka, s naglaskom na nastanak hipertenzije. Preostali nepromjenjivi čimbenici izučavani u ovom radu su spol i dob, a uz njih izučavani su promjenjivi, u širem smislu okolišni, čimbenici: debljina, razina masnoća u krvi i navika pušenja. Pri tome je korišten uzorak izolirane populacije otoka Visa koji se sastoji od 140 osoba u dobi od 40 do 81 godine. Ustanovljena je pozitivna korelacija sistoličkog tlaka s dobi, indeksom tjelesne mase (BMI) i koncentracijom triglicerida, te pozitivna korelacija dijastoličkog tlaka s BMI i koncentracijom triglicerida, a negativna s brojem godina pušenja i indeksom pušenja. Kod muškaraca je značajan utjecaj na povišenje krvnog tlaka imala koncentracija triglicerida, a kod žena je to bio BMI. Budući da ACE I/D polimorfizam nije u kodirajućoj regiji, pretpostavlja se da pokazuje efekt nekog drugog, funkcionalnog polimorfizma, koji je u neravnoteži vezanosti gena s izučavanim. Rezultati su pokazali da se utjecaj polimorfizma ACE gena može objasniti recesivnim modelom, tj. „rizični“ alel utječe na pojavu hipertenzije jedino u homozigotnoj formi. Povećan utjecaj ACE I/D polimorfizma na nastanak hipertenzije utvrđen je nakon uključenja rizičnih čimbenika kao što su spol, dob, BMI, koncentracija kolesterola, HDL kolesterola i triglicerida te indeks pušenja. Međupopulacijskom usporedbom između opće populacije Republike Hrvatske i populacije otoka Visa potvrđena je pogodnost ove izolirane populacije za istraživanja genetičke podloge hipertenzije kao kompleksnog poremećaja.

Abstract (english)

This study aimed to determine whether ACE I/D polymorphism, as a genetic factor located within one of the genes responsible for controlling blood pressure, affects human blood pressure, with an emphasis on hypertension. The remaining factors studied in this work were gender and age as well as, in a broader sense, environmental factors: obesity, fat components in the blood and smoking habit. The sample was drawn from the population isolate of the island of Vis and consisted of 140 persons 40 to 81 years old. A positive correlation was established between systolic pressure and age, BMI and triglyceride concentration, and between the diastolic blood pressure and BMI and triglyceride concentration, as well as a negative correlation between diastolic blood pressure and the number of smoking years and smoking index. A significant influence on the increase of blood pressure in men had the concentration of triglycerides, while in women it was the BMI. Since ACE I/D polymorphism is not located in the coding region of the gene, it was supposed that it showed the effect of another polymorphism that is functional and in the linkage disequilibrium with it. The effect of ACE I/D polymorphism has been shown to obey the recessive model, i.e. the „risk“ allele influenced the development of hypertension only in its homozygous form. The influence of ACE I/D polymorphism on the development of hypertension was found also after taking into account the other risk factors such as gender, age, BMI and the concentrations of cholesterol, HDL and triglycerides as well as smoking index. Comparison between the general population of Croatia and the population of island Vis confirmed the suitability of this isolated population for genetic research of hypertension as a complex disorder.

• Keywords: krvni tlak čovjeka; hipertenzija; ACE I/D polimorfizam; populacijski izolat; otok Vis
• Keywords (english): human blood pressure; hypertension; ACE I/D polymorphism; population isolate; island of Vis
• Language: croatian
• URN:NBN: urn:nbn:hr:217:102807
• Study programme: Title: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-03-16 11:57:19