Određivanje toksičnosti uzoraka sedimenta potoka Gorjak biotestovima in vitro

master's thesis

• Title (english): Determination of sediment toxicity of Gorjak stream by bioassays in vitro
• Author: Ivana Šestak
• Mentor: Tvrtko Smital (mentor)
• Mentor: Goran Klobučar (mentor)
• Committee member: Tvrtko Smital (predsjednik povjerenstva)
• Committee member: Goran Klobučar (član povjerenstva)
• Committee member: Mirjana Pavlica (član povjerenstva)
• Committee member: Željka Vidaković Cifrek (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2009, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Brojna teško razgradiva organska zagađivala (eng. Persistent Organic Pollutants; POPs) poput polikloriranih dibenzo-p-dioksina i furana (PCDD/F), polikloriranih bifenila (PCB), polikloriranih naftalena (PCN) i policikličkih aromatskih ugljikovodika (PAH) izrazito su toksična. POP-sovi u okoliš najčešće dospijevaju industrijskim otpadnim vodama. Ispuštanjem nepročišćenih industrijskih otpadnih voda u potok Gorjak, tvrtke Pliva Hrvatska d.d. i Kvasac d.o.o. desetljećima predstavljaju problem za širu zajednicu. Cilj ovog istraživanja bio je odrediti razinu toksičnosti sedimenta potoka Gorjak pomoću dva biotesta: (1) testa kronične toksičnosti (inhibicija rasta zelene alge Scenedesmus subspicatus); te (2) EROD testa određivanja aktivnosti staničnog detoksikacijskog sustava oksidaza miješanih funkcija. Testom kronične toksičnosti mjerili smo razrjeđenja uzoraka sedimenta pri kojima je dosegnuta 50%-tna inhibicije rasta alge S. subspicatus (IC50) u odnosu na kontrolnu grupu. Odredili smo EC50 vrijednosti koje predstavljaju ekvivalentnu količinu sedimenta, te izračunali toksične jedinice (TJ) dobivene prema izrazu TJ = 100 / EC50. Izvođenjem EROD testa izračunali smo TCDD toksične ekvivalente (TCDD TEQ). Rezultati pokazuju visoku toksičnost sedimenata na svim lokacijama, uz izrazito visoke vrijednosti neposredno nakon ispusta industrijskih otpadnih voda spomenutih tvrtki.

Abstract (english)

Numerous persistent organic pollutants (POPs), such as polychlorinated dibenzo-p-dioxins and furans (PCDD/F), polychlorinated biphenyls (PCBs), polychlorinated naphthalenes (PCNs) and polyaromatic hydrocarbons (PAHs) are highly toxic. POPs get into environment mostly by industrial wastewaters. By releasing untreated industrial wastewater into the Gorjak stream, Industries Pliva Hrvatska d.d. and Kvasac d.o.o. for decades represent a problem for local community. The purpose of this research was to determine ecotoxicity of Gorjak stream sediment samples, using two bioassays: (1) chronic toxicity test (growth inhibition test with green algae Scenedesmus subspicatus), and (2) EROD test for determination of the activity of the mixed function oxygenases cellular detoxification system. Using chronic toxicity test we measured sample dilutions at which 50% growth inhibion of the algae was achieved (IC50) in comparison to the control group. EC50 values representing corresponding amount of sediment were determined and toxicity units (TU) obtained recalculated by expression TU = 100 / EC50. For the EROD bioassay we calculated TCDD toxicity equivalents (TCDD TEQ). Results showed high sediment toxicity at each location, with the highest values determined at location closest to the wastewater outlet of the above mentioned industrial facilities.

• Keywords: POP; biotestovi; kronična toksičnost; EROD; TCDD TEQ
• Keywords (english): POPs; bioassays; chronic toxicity; EROD; TCDD TEQ
• Language: croatian
• URN:NBN: urn:nbn:hr:217:311475
• Study programme: Title: Biology; specializations in: Ecology; Course: Ecology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra biologije (magistar/magistra biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-03-21 09:19:01