Stereološka analiza kriptorhičnih testisa u mladih muškaraca

master's thesis

• Title (english): Stereological analysis of cryptorchid testicles in young males
• Author: Dina Klarin
• Mentor: Ljerka Banek (mentor)
• Committee member: Ljerka Banek (predsjednik povjerenstva)
• Committee member: Gordana Lacković-Venturin (član povjerenstva)
• Committee member: Ines Radanović (član povjerenstva)
• Committee member: Davor Kovačević (član povjerenstva)
• Committee member: Biserka Prugovečki (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2010, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Podaci o građi i broju Leydigovih stanica kod kriptorhičnih testisa mlađih muškaraca vrlo su rijetki. Do sada nije istraženo da li se mijenja broj Leydigovih stanica: a) uz sjemenske kanaliće i b) u preostalom intersticiju testisa uz krvne žile kao i eventualne promjene dužine krvnih žila intersticija. Istraživanje je obuhvatilo stereološku analizu biopsija kriptorhičnih testisa desetorice mladih muškaraca (u dobi od 18 do 27 god.). Kao kontrola poslužili su uzorci tkiva testisa dvojice muškaraca (starih 28 i 29 god.) dobiveni obdukcijom nakon prometne nesreće. Histološki rezovi testisa bojani su hemalaun - eozinom i po Massonu. Dobiveni rezultati istraživanja pokazuju značajno smanjenje (P<0.01) broja Leydigovih stanica pacijenata uz sjemenske kanaliće, dok je broj ovih stanica u preostalom intersticiju uz krvne žile bio značajno povećan (P<0.01) u odnosu na kontrolnu skupinu. Također i broj Reinkeovih kristala u citoplazmi Leydigovih stanica, bez obzira na smještaj, kod kriptorhičnih pacijenata bio je značajno povećan u odnosu na kontrolu (P<0,01). Konačna dužina krvnih žila intersticija kriptorhičnih testisa bila je također povećana u odnosu na kontrolnu skupinu. Promjene u broju Leydigovih stanica koje se nalaze uz sjemenske kanaliće i u preostalom intersticiju vjerojatno su posljedica oštećenja testisa nastalog zbog neadekvatnog položaja testisa. Izrazito povećanje broja Reinkeovih kristala u Leydigovim stanicama kriptorhičnih testisa bez obzira na njihov smještaj u intersticiju vjerojatno je posljedica poremećene funkcije i metabolizma ovih stanica.

Abstract (english)

Information about structure and number of Leydig cells in cryptorchid testicles of younger men are very rare. Until now, it hasn't been examined if the number of Leydig's cells changes: a) along seminiferous tubules and b) in the remaining part of the testicles interstitium beside blood vessels and possible changes in lenght of blood vessels in interstitium. The resarch included a stereological analysis of cryptorchid testicles biopsies of ten young men (aged 18 – 27). As controls, samples of biopsies od two patients (aged 28 and 29) who had a car acident, were used. Histological cuts of testicles were stained with hemalauneosin and by Masson. The results of this research show significant reduction (P<0.01) of patient's Ledig's cells along seminiferous tubules, while the number of cells in the remaining part of the interstitium beside blood vessels was significantly enlarged (P<0.01) by comparison with the control group. Also the number of Reinke's crystals in the cytoplasm of Ledig's cells, regardless of the location, with cryptorchid patients was significantly enlarged by comparison with the control (P<0,01). The ultimate lenght of blood vessles of interstitium in cryptorchid testicles was also enlarged in relation to the control group. Change in the number of Ledig's cells along seminiferous tubules and in the rest of the interstitium are probably the result of testicles damage which appeared because of inadequate placement od testicles. Extreme increase in number of Reinke's crystals in Ledig's cells of cryptorchid testicles regardles of their location in the interstitium is probably the result of disrupted function and metabolism of these cells.

• Keywords: testis; Reinkeovi kristali; kriptorhizam; stereologija
• Keywords (english): testicles; Reinke's crystals; cryptorchidism; stereology
• Language: croatian
• URN:NBN: urn:nbn:hr:217:115818
• Study programme: Title: Biology and Chemistry; Study programme type: university; Study level: graduate; Academic / professional title: profesor biologije i kemije (profesor biologije i kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-03-26 08:21:25