Prooksidativni učinak kvercetina na vrstu Polycelis felina (Daly.)

master's thesis

• Title (english): Prooxidative effect of quercetin on the species Polycelis felina (Daly.)
• Author: Helena Matković
• Mentor: Mirjana Kalafatić (mentor)
• Committee member: Mirjana Kalafatić (predsjednik povjerenstva)
• Committee member: Ana Galov (član povjerenstva)
• Committee member: Renata Šoštarić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2010, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Flavonoidi su fenolni spojevi široko rasprostranjeni u biljkama kao sekundarni metaboliti. Posjeduju širok spektar bioloških učinaka, a ti učinci posljedica su moduliranja djelovanja enzima i nuklearnih receptora, utjecaja na ekspresiju gena, te helatiranja metalnih iona. Kvercetin je najčešći flavonoid u biljkama i najviše je proučavan. Do sada nije proučavan učinak kvercetina na vrstu Polycelis felina (Daly.) u laboratorijskim uvjetima, koja je pogodan test organizam u ovakvim istraživanjima. U ovim pokusima utvrđeno je da kvercetin u primijenjenim koncentracijama izaziva promjene u ponašanju tretiranih životinja, morfološke promjene u obliku depigmentacije pojedinih dijelova tijela, te promjene u histološko-citološkoj građi tretiranih planarija. Najizrazitije promjene uočavaju se na totipotentnim stanicama neoblastima, te na retikularnim stanicama. Utvrđene promjene bile su najintezivnije trećeg dana nakon tretiranja, a spomenute promijenjene stanice bile se zastupljene u većem broju u odnosu na kontrolnu skupinu jedinki.

Abstract (english)

Flavonoids are phenolic compounds widely present in plants as secondary metabolites. They exhibit a wide range of biological effects arising mainly from their ability to modulate several enzymes or nuclear receptors, alter gene expression, chelate bivalent metals. Quercetin is the most abundant of the flavonoid molecules in plants and it is the most studied one. Quercetin effect on Polycelis felina (Daly.), which is convenient test organism in such studies, has not been analyzed in laboratory yet. In these tests it is determined that implemented concentration of quercetin cause behevioral changes of treated animals, morphological changes in depigmentation of certain body parts and histopathological-cytological structure of treated planarian. The most exceptional changes are noticed on totipotent cells of neoblasts and reticular cells. Determined changes were most intensive on the third day after treatment, and above mentioned cells were represented in higher number compared to control group of animals.

• Keywords: kvercetin; flavonoid; depigmentacija; kontrakcije
• Keywords (english): qercetin; flavonoid; depigmentation; contractions
• Language: croatian
• URN:NBN: urn:nbn:hr:217:195487
• Study programme: Title: Biology; specializations in: Ecology; Course: Ecology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra biologije (magistar/magistra biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-03-28 09:58:13