Stabilnost N-glikoma ljudske plazme

master's thesis

• Title (english): Human plasma N-glycome stability
• Author: Marina Mavrinac
• Mentor: Gordan Lauc (mentor)
• Committee member: Mirjana Pavlica (predsjednik povjerenstva)
• Committee member: Željka Vidaković Cifrek (član povjerenstva)
• Committee member: Dubravka Hranilović (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2010, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

N-glikani su šećerne komponente glikoproteina čije su biološke funkcije za organizam vrlo važne te ponekad i esencijalne. Kod raznih bolesti (tumori, urođeni poremećaji glikozilacije, upalne bolesti) i stanja (alkoholizam), strukture glikana mogu biti izmijenjene, što može dovesti do funkcionalnih promjena glikoproteina. Dijagnostički potencijal glikanskih struktura je vrlo velik, međutim, da bi se omogućilo korištenje glikana kao bioloških markera, potrebno je ispitati dolazi li do tih promjena i u zdravom organizmu pri uobičajenim fiziološkim uvjetima. Tekućinskom kromatografijom visoke djelotvornosti ispitana je stabilnost glikana ljudske plazme na uzorku od 12 zdravih osoba (6 muškaraca i 6 žena). Dobiveni rezultati pokazali su vrlo veliku stabilnost N-glikoma unutar kraćeg perioda, što daje čvrstu osnovu za daljnja istraživanja N-glikana u smjeru korištenja istih u dijagnostici.

Abstract (english)

N-glycans are carbohydrate parts of glycoproteins that play very important and sometimes even essential biological roles in an organism. In various diseases (tumors, congenital disorders of glycosylation, inflamation) and conditions (alcoholism), glycan structures can be changed, which can lead to functional changes of glycoproteins. Diagnostic potential of glycan structures is very high, but, in order to provide the use of glycans as biomarkers, it is necessary to examine if these changes occur in a healthy individual during normal phisiological conditions. Stability of human plasma N-glycome was examined by high performance liquid chromatography in 12 healthy individuals (6 males and 6 females). Results obtained in this research show very high N-glycome stability during a short period of time, which gives a firm basis for future research aiming to use glycans in diagnostics.

• Keywords: strukture glikana; poremećaji glikozilacije; biološki markeri; dijagnostika
• Keywords (english): glycan structures; disorders of glycosylation; biomarkers; diagnostics
• Language: croatian
• URN:NBN: urn:nbn:hr:217:237353
• Study programme: Title: Biology; specializations in: Molecular biology; Course: Molecular biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra biologije (magistar/magistra biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-03-28 10:04:01