Genotoksični učinci sevoflurana i izoflurana na stanice bubrega miša Swiss albino

master's thesis

• Title (english): Genotoxic effects of sevoflurane and isoflurane on kidney cells in Swiss albino mice
• Author: Darko Pavlić
• Mentor: Gordana Brozović (mentor)
• Mentor: Nada Oršolić (sumentor)
• Committee member: Nada Oršolić (član povjerenstva)
• Committee member: Višnja Besendorfer (član povjerenstva)
• Committee member: Damjan Franjević (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2011, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

U radu je istraživano oštećenje DNA stanica bubrega miša Swiss albino nakon višestrukog izlaganja inhalacijskim anesteticima sevofluranu i izofluranu. Za procjenu oštećenja DNA upotrijebljen je alkalni komet test, a praćena su 3 parametra: duljina repa (TL), repni moment (TM) i intenzitet repa (TI). Stupanj oštećenja DNA određivan je neposredno nakon izlaganja sevofluranu, odnosno izofluranu, te nakon 2 h, 6 h i 24 h. Srednje vrijednosti sva 3 parametra bile su statistički značajno više u eksperimentalnim skupinama u odnosu na kontrolne skupine, s tim da je kod miševa izloženih sevofluranu utvrđen kontinuiran porast oštećenja DNA unutar 24 h nakon izlaganja, dok je kod izoflurana najveće oštećenje opaženo 2 sata nakon izlaganja, nakon čega je došlo do popravka.

Abstract (english)

DNA damage of kidney cells in Swiss Albino mice after repeated exposure to inhalation anesthetics sevoflurane and isoflurane has been investigated. For evaluation of DNA damage alkaline comet assay has been used. Three parameters have been measured – tail length (TL), tail moment (TM) and tail intensity (TI), immediately after exposure to sevoflurane and isoflurane, as well as 2 h, 6 h and 24 h after exposure. Mean values of all three parameters have been significantly higher in experimental groups compared to the control groups. DNA damage in mice exposed to sevoflurane increased continuously within 24 h after exposure, while isoflurane induced highest DNA damage 2 h after exposure and afterwards DNA repair was observed.

• Keywords: bubreg; genotoksicnost; inhalacijski anestetici; izofluran; komet test; sevofluran; oštećenje DNA
• Keywords (english): comet assay; DNA damage; genotoxicity; inhalation anesthetics; isoflurane; kidney; sevoflurane
• Language: croatian
• URN:NBN: urn:nbn:hr:217:470148
• Study programme: Title: Biology; specializations in: Molecular biology; Course: Molecular biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra biologije (magistar/magistra biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-04-09 11:48:31