Regulacija aktivnosti urokinaznog sustava u ljudskim stanicama glioblastoma

master's thesis

• Title (english): Regulation of urokinase activation system in human glioblastoma cells
• Author: Lidija Požgaj
• Mentor: Maja Matulić (mentor)
• Mentor: Josip Madunić (pomoć pri izradi, neposredni voditelj)
• Committee member: Maja Matulić (član povjerenstva)
• Committee member: Mirta Tkalec (član povjerenstva)
• Committee member: Nada Oršolić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2011, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Sustav aktivacije plazminogena proteolitički je sustav koji ima veliku ulogu u fiziološkim i patološkim procesima u kojima dolazi do pregradnje izvanstaničnog matriksa. Sustav aktivacije plazminogena reguliran je na razini ekspresije urokinaznog i tkivnog tipa plazminogenskog aktivatora (uPA i tPA), inhibitora plazminogenskog aktivatora (PAI-1 i PAI-2) i receptora za uPA (uPAR). Osnovni je princip djelovanja sustava aktivacija plazminogena i nastanak plazmina, proteaze širokog spektra djelovanja. Cilj ovog istraživanja bio je istraživanje mehanizma regulacije sustava plazminogenske aktivacije kod stanica glioblastoma A1235, nakon alkilacijskog oštećenja, te je li u te procese uključena aktivacija MAP-kinaze JNK. Stanična linija glioblastoma A1235, kojoj nedostaje ekspresija enzima popravka O6metilguanin-metiltransferaze, nakon alkilirajućeg oštećenja inducira porast urokinazne aktivnosti. Inhibitor JNK nije pokazao znatnu inhibiciju indukcije aktivnosti uPA u stanicama A1235, nakon alkilirajućeg oštećenja. Sva ova istraživanja ukazuju na to da je regulacija urokinazne aktivnosti složen sustav, a signalni putevi indukcije ovisni o tipu stanica.

Abstract (english)

Plasminogen activation system is a proteolytic system that plays an important role in physiological and pathological processes in which extracellular matrix remodelling occurs. Plasminogen activation system is regulated on the level of transcription of urokinase-type and tissue-type plasminogen activator (uPA and tPA), plasminogen activator inhibitors (PAI-1 i PAI-2) and uPA receptor (uPAR). The aim of this study was to investigate the regulation of urokinase activation system in A1235 human glioblastoma cells after alkylation treatment and to determine whether this induction of urokinase activity was associated with activation of JNK MAP-kinase. A1235 cells, lacking the expression of the O6metilguanin methyltransferase, after alkylation damage induced increase in urokinase activity. Inhibitor JNK didn´t show significant inhibition of uPA activity in A1235 cells after alkylation damage. The experiments indicate the existance of complex pathways regulating urokinase activity, showing the cell type specificity.

• Keywords: uPA; stanična linija A1235; alkilacijsko oštećenje; JNK
• Keywords (english): uPA; cell line A1235; alkylation damage; JNK
• Language: croatian
• URN:NBN: urn:nbn:hr:217:236261
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-04-10 09:15:23