Analiza unutarsortne varijabilnosti vinove loze upotrebom retrotranspozonskih biljega

master's thesis

• Title (english): Analysis of grapevine intravarietal polymorphism using retrotranspone-based markers
• Author: Jelena Rajič
• Mentor: Višnja Besendorfer (mentor)
• Mentor: Nenad Malenica (pomoć pri izradi, neposredni voditelj)
• Committee member: Željka Vidaković Cifrek (član povjerenstva)
• Committee member: Damjan Franjević (član povjerenstva)
• Committee member: Ivan Pejić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2011, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Vinova loza (Vitis vinifera sp.) jedna je od najvažnijih voćnih kultura zastupljena s oko 10 000 sorti, a unutar kojih razlikujemo brojne klonove. Klonska selekcija vinove loze izdvaja one klonove koji pokazuju značajna odstupanja u fenotipu, a posljedica su nekih genetičkih promjena između tih klonova. Moguća objašnjenja za varijabilnost klonova su: poliklonalno porijeklo sorti, infekcije patogenima, somatske mutacije, epigenetičke promjene i kimerizam. U ovom radu pomoću tehnika IRAP i REMAP istražena je unutarsortna varijabilnost klonova hrvatske autohtone sorte Plavac mali, kao i genetička osnova promjene boje kožice u Plavcu malom sivom. Navedene tehnike koriste se retrotranspozonskim insercijama kao izvorom DNA polimorfizama. Obje se temelje na lančanoj reakciji polimerazom (PCR) za umnaţanje mjesta ugradnje retrotranspozona u genomskoj DNA. Detektirani polimorfizmi mogli bi se koristiti za razvoj DNA biljega u svrhu rane identifikacije klonova s korisnim/superiornim agronomskim svojstvima. Istraživanje je pokazalo relativno mali broj polimorfizama među klonovima, koji su se samo djelomično mogli dovesti u vezu s njihovom iskazanom fenotipskom raznolikosti. Međutim, upotrebljene tehnike su se pokazale kao brz, jednostavan i pouzdan način za preliminarno razlikovanje sorti vinove loze.

Abstract (english)

Grapevine (Vitis vinifera sp.) is one of the most important fruit cultures represented with approximately 10 000 different varieties which are constituted of numerous clones. Clonal selection of grapevine singles out those clones that show significant deviations in phenotype, resulting from some genetic changes between these clones. Possible explanations of clonal variability are the following: polyclonal origin of cultivars, phatogen infections, somatic mutations, epigenetics and chimerism. In this work we studied clonal variation of the Croatian autochthonous cultivar Plavac mali by REMAP and IRAP techniques as well as genetic basis of skin color changes in Plavac mali sivi. These techniques use insertions of retrotransposons as a source of DNA polymorphisms. Both are based on polymerase chain reaction (PCR), which is used for amplification retrotransposon insertion sites in genomic DNA. Detected polymorphisms could be used for developing DNA markers for early identification of clones with useful / superior agronomic traits. A relatively small number of polymorphisms are detect between the clones, which could only partially be correlated with their expressed phenotypic variation. However, applied techniques have proven to be fast, easy and cheap way to preliminary distinguish grape varieties.

• Keywords: vinova loza; retrotranspozoni; IRAP; REMAP
• Keywords (english): grapevine; retrotransposons; IRAP; REMAP
• Language: croatian
• URN:NBN: urn:nbn:hr:217:151795
• Study programme: Title: Biology; specializations in: Molecular biology; Course: Molecular biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra biologije (magistar/magistra biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-04-10 10:51:36